Chromene suppresses activated inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells

Title
Chromene suppresses activated inflammatory mediators in lipopolysaccharide-stimulated RAW 264.7 cells
Author(s)
장지이; 예보람; 김준성; 김민선; 김지형; 이영득; 이수진; 오철홍; 강도형; 허수진
KIOST Author(s)
Kim, Junseong(김준성)Lee, Youngdeuk(이영득)Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)Heo, Soo Jin(허수진)
Publication Year
2013-06-27
Abstract
Inflammation is complex process in which a variety of immune cells are involved to defense from certain stimuli in the mammal’s body. However, it has been reported that over generated pro-inflammatory cytokines and mediators could get worse as disease or cancer. To find natural effective remedial agent for inflammation, in this study, we isolated functional algal compound from Sargassum siliquastrum, collected from Jeju Island of Korea, which were named as sargachromanol D (SD), a kind of chromene. And then we evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells through determining cell viability, cytotoxicity, production of inflammatory mediators (NO, PGE2, iNOS, COX-2), and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6). As a result, SD inhibited production of nitric oxide (NO) and prostaglandin E2 (PGE2) on LPS-induced cells by preventing the expression of inflammatory mediators such as inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Concurrently, cytokines like TNF-α, IL-1β, and IL-6 causing inflammatory response were gradually restrained according to increasing concentration of SD. In addition, SD played a role in frustrating the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These findings sue as disease or cancer. To find natural effective remedial agent for inflammation, in this study, we isolated functional algal compound from Sargassum siliquastrum, collected from Jeju Island of Korea, which were named as sargachromanol D (SD), a kind of chromene. And then we evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells through determining cell viability, cytotoxicity, production of inflammatory mediators (NO, PGE2, iNOS, COX-2), and pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6). As a result, SD inhibited production of nitric oxide (NO) and prostaglandin E2 (PGE2) on LPS-induced cells by preventing the expression of inflammatory mediators such as inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) in a dose-dependent manner. Concurrently, cytokines like TNF-α, IL-1β, and IL-6 causing inflammatory response were gradually restrained according to increasing concentration of SD. In addition, SD played a role in frustrating the activation of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26886
Bibliographic Citation
KSABC, pp.207, 2013
Publisher
한국응용생명화학회
Type
Conference
Language
English
Publisher
한국응용생명화학회
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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