Identification of gene expressions in different body regions of the hydrothermal vent shrimp Nautilocaris saintlaurentae by the environmental changes

Abstract

Deep-sea invertebrates are known to tolerate a wide range of temperatures and pressures. However, few studies have been conducted to identify gene expression profiles in vent organisms for environmental stressors. The hydrothermal vent shrimp, Nautilocaris saintlaurentae, were collected using a suction sampler mounted on ROV (MAGNUM Oceaneering International, Inc.) at a hydrothermal vent field in Tofua Arc (1018m deep), the SW Pacific. Three commonly known stress-related genes, Hsp70, Hsp90, and Glutathione peroxidase (GPx), were partially sequenced for this shrimp. Then primer pairs were specifically designed and tested their stability in qRT-PCR assays. The expression of three genes was examined from two body parts (cephalothorax and abdomen) of individuals incubated at 4°C for 72 hours on board. Overall, the expression of Hsp70 and Hsp90 was up-regulated in the early (~24 h), and after the maximum expression, they showed a tendency to down-regulation. In the early stage of stress response, expression of chaperone proteins was quickly progressed to protect protein degradation and to assist structure stabilization of other proteins. However, in abdomen, Hsp70 showed a very-low-expression. By contrast, Hsp90 was remarkable over-expressed at 12 h. These actions suggested that N. saintlaurentae improved the efficiency of energy consumption for maintaining muscle cells in abdomen because the abdomen region is mainly cmp, Nautilocaris saintlaurentae, were collected using a suction sampler mounted on ROV (MAGNUM Oceaneering International, Inc.) at a hydrothermal vent field in Tofua Arc (1018m deep), the SW Pacific. Three commonly known stress-related genes, Hsp70, Hsp90, and Glutathione peroxidase (GPx), were partially sequenced for this shrimp. Then primer pairs were specifically designed and tested their stability in qRT-PCR assays. The expression of three genes was examined from two body parts (cephalothorax and abdomen) of individuals incubated at 4°C for 72 hours on board. Overall, the expression of Hsp70 and Hsp90 was up-regulated in the early (~24 h), and after the maximum expression, they showed a tendency to down-regulation. In the early stage of stress response, expression of chaperone proteins was quickly progressed to protect protein degradation and to assist structure stabilization of other proteins. However, in abdomen, Hsp70 showed a very-low-expression. By contrast, Hsp90 was remarkable over-expressed at 12 h. These actions suggested that N. saintlaurentae improved the efficiency of energy consumption for maintaining muscle cells in abdomen because the abdomen region is mainly c