Characterization of BacteriaI Diversitν and Pathogenic Bacteria in the Water Masses fron Green island, Taiwan Usi ng Pyrosequencing

Abstract

The investigation of patterns of microbial diversity and composition from seawater is very important to understand the roles of microbes in modifications to the basic biological properties of global marine organisms. Furthermore, it could be foundation for further studies to determine the symbiotic relationship between pathogens and their hosts. Although the potential roles of microbes in marine ecosystem have been identified, their diversity and interactions with environmental factors such as temperature, salinity, pH and light transmittance are still unclear. So, we exploited a 16S rRNA gene taq-pyrosequencing technique to define the bacterial communities associated with seawater samples in green island, Taiwan. Our results showed that about 1154 Operational Taxonomic Units (OTUs) were found from seawater samples, and bacterial 25 phyla were detected. Among them, Proteobacteria was identified as a most dominant group, representing at least 82% of the total population. Halomonas, Tenacibaculum, Vibrio and Pseudoalteromonas were found as pathogenic bacteria from seawater samples, of which Vibrios were dominant.e foundation for further studies to determine the symbiotic relationship between pathogens and their hosts. Although the potential roles of microbes in marine ecosystem have been identified, their diversity and interactions with environmental factors such as temperature, salinity, pH and light transmittance are still unclear. So, we exploited a 16S rRNA gene taq-pyrosequencing technique to define the bacterial communities associated with seawater samples in green island, Taiwan. Our results showed that about 1154 Operational Taxonomic Units (OTUs) were found from seawater samples, and bacterial 25 phyla were detected. Among them, Proteobacteria was identified as a most dominant group, representing at least 82% of the total population. Halomonas, Tenacibaculum, Vibrio and Pseudoalteromonas were found as pathogenic bacteria from seawater samples, of which Vibrios were dominant.