Development of a Nile Red staining method for microplastic identification and quantification

Title
Development of a Nile Red staining method for microplastic identification and quantification
Author(s)
송영경; 심원준; 홍상희; 장미; 한기명
KIOST Author(s)
Song, Young Kyoung(송영경)Shim, Won Joon(심원준)Hong, Sang Hee(홍상희)Jang, Mi(장미)Han, Gi Myung(한기명)
Publication Year
2014-01-13
Abstract
Nile Red (NR), a fluorescent dye, which was well known to dye neutral lipid in cell and tissuesample. It is strongly fluorescent only in the presence of a hydrophobic environment. Applicabilityof NR for identification and quantification of microplastics was tested and stainingcondition was optimized in this study. Micro-sized polyethylene (PE), polypropylene (PP) andexpanded polystyrene (EPS) were used as model plastics. A number of carrying solvents weretested. NR demonstrated good staining capability in n-hexane without damage on both whiteand black polycarbonate (PC) filter papers. But, because low solubility of NR in n-hexane,stock solution was made in acetone and then diluted with n-hexane in working solution (5 and50 mg/L). Stained polymer particles were recognized better in green fluorescence (Ex. 534-558and Em. 515-565) than red (Ex. 534-558 and Em. > 590) without background (PC filterpaper) staining under a fluorescent microscope. Both the NR concentrations were enough todye three polymer particles, but 50 mg/L solution showed higher fluorescence than 5 mg/L solution.Fluorescence intensity was in the order of EPS > PP > PE. The developed NR stainingmethod was successfully applied to identify and quantify the fragmented polymer particles in alaboratory accelerated mechanical abrasion study. Staining polymer particles with NR is easyand fast to identify and quantify polymer pa of microplastics was tested and stainingcondition was optimized in this study. Micro-sized polyethylene (PE), polypropylene (PP) andexpanded polystyrene (EPS) were used as model plastics. A number of carrying solvents weretested. NR demonstrated good staining capability in n-hexane without damage on both whiteand black polycarbonate (PC) filter papers. But, because low solubility of NR in n-hexane,stock solution was made in acetone and then diluted with n-hexane in working solution (5 and50 mg/L). Stained polymer particles were recognized better in green fluorescence (Ex. 534-558and Em. 515-565) than red (Ex. 534-558 and Em. > 590) without background (PC filterpaper) staining under a fluorescent microscope. Both the NR concentrations were enough todye three polymer particles, but 50 mg/L solution showed higher fluorescence than 5 mg/L solution.Fluorescence intensity was in the order of EPS > PP > PE. The developed NR stainingmethod was successfully applied to identify and quantify the fragmented polymer particles in alaboratory accelerated mechanical abrasion study. Staining polymer particles with NR is easyand fast to identify and quantify polymer pa
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26450
Bibliographic Citation
International workshop fate and impact of microplastics in marine ecosystems, pp.57, 2014
Publisher
International workshop fate and impact of microplastic in marine ecosystems
Type
Conference
Language
English
Publisher
International workshop fate and impact of microplastic in marine ecosystems
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse