DNA바코드를 이용한 특정 동물플랑크톤과 유생의 종출현 탐지

Title
DNA바코드를 이용한 특정 동물플랑크톤과 유생의 종출현 탐지
Alternative Title
Detecting the occurrence of specific target zoo plankton and larvae using DNA barcode
Author(s)
정다금; 정길아; 이유철; 김하나; 강미혜; 최희정; 명정구; 이윤호
KIOST Author(s)
Choi, Hee Jung(최희정)Myoung, Jung Goo(명정구)Lee, Youn Ho(이윤호)
Publication Year
2014-04-22
Abstract
Evaluation of species occurrence and recruitment is an important step toward biodiversity assessment and is a necessary procedure in the studies of biogeography, conservation biology and ecology. Monitoring of the distribution and recruitment through identification of the larvae becomes important for the establishment of proper resource management and conservation plans.On the other hand, it is often difficult to identify plankton species due to their small size and indistinguishable morphological characters. Here we present a molecular approach based on the DNA barcode to detect presence of a target species in the multi-species mixed samples. We developed species-specific primers and probes for marine invertebrates common in the Korean waters which can amplify mitochondrial DNA sequence in the 5 region of the COI gene and distinguish the presence of each species in the netted samples. The horizontal and vertical sampling using 200um and 60um net were carried out from the Tongyung waters twice a month in 2013. Through the realtime-PCR with the species specific primer sets, the presence of the target species such as Paracalanus parvus, Calanus sinicus and other invertebrates in the mixed net samples was examined and also verified by the result of next generation sequencing.Enabling species identification from the mixed net samples, this molecular technique using the DNA barcode opens a new perspective for thent through identification of the larvae becomes important for the establishment of proper resource management and conservation plans.On the other hand, it is often difficult to identify plankton species due to their small size and indistinguishable morphological characters. Here we present a molecular approach based on the DNA barcode to detect presence of a target species in the multi-species mixed samples. We developed species-specific primers and probes for marine invertebrates common in the Korean waters which can amplify mitochondrial DNA sequence in the 5 region of the COI gene and distinguish the presence of each species in the netted samples. The horizontal and vertical sampling using 200um and 60um net were carried out from the Tongyung waters twice a month in 2013. Through the realtime-PCR with the species specific primer sets, the presence of the target species such as Paracalanus parvus, Calanus sinicus and other invertebrates in the mixed net samples was examined and also verified by the result of next generation sequencing.Enabling species identification from the mixed net samples, this molecular technique using the DNA barcode opens a new perspective for the
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26343
Bibliographic Citation
IOC/WESTPAC, 9th International Scientific Symposium, pp.200 - 201, 2014
Publisher
IOC/WESTPAC
Type
Conference
Language
English
Publisher
IOC/WESTPAC
Related Researcher
Research Interests

Marine Molecular Genetics,Marine Ecology and Evolution,해양분자유전,해양생태및진화

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