Novel aerobic and thermophilic bacteria enriched with Macromolecular Carbohydrates

Abstract

Diverse microorganisms are reported from marine environment, displaying various physico-chemical ecosystems which are three dimensional, however, many of which still not identified. Thermophilic bacteria were enriched with 0.1% of lignin or cellulose at 50 OC. The 74 isolates affiliated with Bacillales (65%), Proteobacteria (16%), Actinobacteria (8%), Bacteroidetes (7%), and Archaea (4%) and approx. 20% of isolates constitutes novel taxon. Some of the isolates closely related with previously reported thermophiles but some has been not known as thermophilic or thermotolerants. Amongst, four isolates produces deep branch in each phylogenetic group shows highest similarity with Caldalkalibacillus uzonensis (MEBiC 09487, 89%), Mechercharimyces asporophorigenens (MEBiC 07976, 91%), Pararhodobacter aggregans (MEBiC 09520, 90.7%), or Rhodothermus profundi (MEBiC 09517, 88%). The four isolates were neutrophilic and halophilic and showed 50 OC as optimum growth temperature. The results implied that novel microorganisms could be enriched by combination of high temperature and recalcitrant substrates. cellulose at 50 OC. The 74 isolates affiliated with Bacillales (65%), Proteobacteria (16%), Actinobacteria (8%), Bacteroidetes (7%), and Archaea (4%) and approx. 20% of isolates constitutes novel taxon. Some of the isolates closely related with previously reported thermophiles but some has been not known as thermophilic or thermotolerants. Amongst, four isolates produces deep branch in each phylogenetic group shows highest similarity with Caldalkalibacillus uzonensis (MEBiC 09487, 89%), Mechercharimyces asporophorigenens (MEBiC 07976, 91%), Pararhodobacter aggregans (MEBiC 09520, 90.7%), or Rhodothermus profundi (MEBiC 09517, 88%). The four isolates were neutrophilic and halophilic and showed 50 OC as optimum growth temperature. The results implied that novel microorganisms could be enriched by combination of high temperature and recalcitrant substrates.