Pleiona flava sp. nov., isolated from the sediment of Daebu-Island in the Yellow sea, Korea

Abstract

A gram negative, non-motile, yellow pigmented and rod shaped strain, MEBiC 06907 T, was isolated from a sediment of Daebu-Island in the Yellow sea, Korea. Strain MEBiC 06907T was oxidase positive and catalase negative. The strain is mesophilic, neutrophilic, and halophilic (require seawater component for growth). The 16S rRNA gene sequence analysis (98.1% similarity with Pleionea mediterranea DSM 25350T) and DNA-DNA hybridization (26.12% with P. mediterranea DSM 25350T) revealed that strain MEBiC 06907T is a novel member of the genus Pleionea. The predominant cellular fatty acids were i-C14:0, i-C15:0, i-C16:0, i-C17:0, and summed feature 9 (comprised of C16:0 10-methly and/or C17:1ω9c). The DNA G+C contents is 46.7 mol%. The respiratory quinone is Q-8. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid, unidentified glycolipid, unidentified aminolipid and three unidentified aminophospholipids. On the basis of this polyphasic taxonomic data, strain MEBiC 06907T should be classified as a novel species in the genus Pleionea, and proposed as Pleiona flava sp. nov. The type strain is MEBiC 06907T.lic, neutrophilic, and halophilic (require seawater component for growth). The 16S rRNA gene sequence analysis (98.1% similarity with Pleionea mediterranea DSM 25350T) and DNA-DNA hybridization (26.12% with P. mediterranea DSM 25350T) revealed that strain MEBiC 06907T is a novel member of the genus Pleionea. The predominant cellular fatty acids were i-C14:0, i-C15:0, i-C16:0, i-C17:0, and summed feature 9 (comprised of C16:0 10-methly and/or C17:1ω9c). The DNA G+C contents is 46.7 mol%. The respiratory quinone is Q-8. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol, unidentified phospholipid, unidentified glycolipid, unidentified aminolipid and three unidentified aminophospholipids. On the basis of this polyphasic taxonomic data, strain MEBiC 06907T should be classified as a novel species in the genus Pleionea, and proposed as Pleiona flava sp. nov. The type strain is MEBiC 06907T.