Comparison of CO-dependent H2 Production with Strong Promoters in Thermococcus onnurineus NA1
DC Field | Value | Language |
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dc.contributor.author | 이성혁 | - |
dc.contributor.author | 김민식 | - |
dc.contributor.author | 배승섭 | - |
dc.contributor.author | 최애란 | - |
dc.contributor.author | 이진원 | - |
dc.contributor.author | 김태완 | - |
dc.contributor.author | 이정현 | - |
dc.contributor.author | 이현숙 | - |
dc.contributor.author | 강성균 | - |
dc.date.accessioned | 2020-07-16T04:52:19Z | - |
dc.date.available | 2020-07-16T04:52:19Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2014-05-02 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/26296 | - |
dc.description.abstract | To overproduce biotechnologically valuable products, the expression level of target genes has been modulated by using strong promoters. In a hyperthermophilic archaeon Thermococcus onnurineus NA1, two promoters, PTN0413 and PTN0157, which drive expression of the genes encoding the Slayer protein and glutamate dehydrogenase were inserted in front of a gene cluster encoding a carbon monoxide dehydrogenase, a hydrogenase and a Na+/H+ antiporter. Two promoters exhibited strong activity by increasing the transcription and translation levels of the gene cluster in the mutant strains by 2.5- to 49-folds and 1.4- to 3.3-folds, respectively, than the native promoter in the wild-type strain. While KS0413 with PTN0413 promoter exhibited 2.7 to 4.7 times higher transcript level than KS0157 with PTN0157 promoter, the levels of proteins were a little different between them. The biomass concentrations and H2 production rates of two mutants were 2- to 3-fold higher than those of the wild-type strain in a bioreactor where CO was supplied at a flow rate of 120 ml min− 1. Two mutants showed differential response to the higher CO flow rate, 240 ml min− 1, in terms of growth pattern and product formation, indicating two promoters were regulated by culture conditions. The results demonstrate that not only promoter strength but also product-forming conditions should be considered in promoter engineering.rive expression of the genes encoding the Slayer protein and glutamate dehydrogenase were inserted in front of a gene cluster encoding a carbon monoxide dehydrogenase, a hydrogenase and a Na+/H+ antiporter. Two promoters exhibited strong activity by increasing the transcription and translation levels of the gene cluster in the mutant strains by 2.5- to 49-folds and 1.4- to 3.3-folds, respectively, than the native promoter in the wild-type strain. While KS0413 with PTN0413 promoter exhibited 2.7 to 4.7 times higher transcript level than KS0157 with PTN0157 promoter, the levels of proteins were a little different between them. The biomass concentrations and H2 production rates of two mutants were 2- to 3-fold higher than those of the wild-type strain in a bioreactor where CO was supplied at a flow rate of 120 ml min− 1. Two mutants showed differential response to the higher CO flow rate, 240 ml min− 1, in terms of growth pattern and product formation, indicating two promoters were regulated by culture conditions. The results demonstrate that not only promoter strength but also product-forming conditions should be considered in promoter engineering. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국미생물학회 | - |
dc.relation.isPartOf | 한국미생물학회 국제학술대회 | - |
dc.title | Comparison of CO-dependent H2 Production with Strong Promoters in Thermococcus onnurineus NA1 | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 151 | - |
dc.citation.startPage | 151 | - |
dc.citation.title | 한국미생물학회 국제학술대회 | - |
dc.contributor.alternativeName | 이성혁 | - |
dc.contributor.alternativeName | 김민식 | - |
dc.contributor.alternativeName | 배승섭 | - |
dc.contributor.alternativeName | 최애란 | - |
dc.contributor.alternativeName | 김태완 | - |
dc.contributor.alternativeName | 이정현 | - |
dc.contributor.alternativeName | 이현숙 | - |
dc.contributor.alternativeName | 강성균 | - |
dc.identifier.bibliographicCitation | 한국미생물학회 국제학술대회, pp.151 | - |
dc.description.journalClass | 2 | - |