DIFFERENT PRIMARY TOXIC SENSITIVITY ON EMBRYONIC FISH EXPOSED TO IRANIAN HEAVY CRUDE OIL MALFORMATION AND CYP1A EXPRESSIONY

Title
DIFFERENT PRIMARY TOXIC SENSITIVITY ON EMBRYONIC FISH EXPOSED TO IRANIAN HEAVY CRUDE OIL MALFORMATION AND CYP1A EXPRESSIONY
Author(s)
채영선; 김하나; 심원준; 정지현
KIOST Author(s)
Shim, Won Joon(심원준)Jung, Jee Hyun(정지현)
Publication Year
2014-05-12
Abstract
In the study, to clarify theeffects of spilled oil on resident fish development, spotted bass(Lateolabrax maculates) and olive flounder (Paralichthys olivaceus)embryos (48 hr post-fertilization) were exposed to oiled gravel effluentof Iranian Heavy crude oil (IHCO) oil for 48 hr. Embryos weremanually dechorionated and observed by a stereomicroscope and acompound microscope. Embryos were fixed in 4% phosphate-bufferedparaformaldehyde and stained for CYP1A and myosin heavy chain.After 24 hr, several complex effects, including the pericardium edemaand tail mutant were observed in both of embryos after the IHCOexposure. The distribution of CYP1A in embryo was assessed byimmunofluorescence. CYP1A immunofluorescence activity was highsignificantly in the endothelium of head, epidermis and cardium and asignificant induction of CYP1A mRNA was observed in both ofembryos after the oil exposure. The extent of tail abnormalities andedema in olive flounder were stronger than those of spotted bass in oilexposed groups. But the levels of CYP1A expression were lower thanthose of spotted bass. Our results confirm that the HSOS causedsignificant damage to resident fish embryos and different toxicsensitivity were investigated in both species at morphological andbiochemical levels. This study was supported by research fund from theKorean Ministry of Land, Transport and Maritime Affairsof Iranian Heavy crude oil (IHCO) oil for 48 hr. Embryos weremanually dechorionated and observed by a stereomicroscope and acompound microscope. Embryos were fixed in 4% phosphate-bufferedparaformaldehyde and stained for CYP1A and myosin heavy chain.After 24 hr, several complex effects, including the pericardium edemaand tail mutant were observed in both of embryos after the IHCOexposure. The distribution of CYP1A in embryo was assessed byimmunofluorescence. CYP1A immunofluorescence activity was highsignificantly in the endothelium of head, epidermis and cardium and asignificant induction of CYP1A mRNA was observed in both ofembryos after the oil exposure. The extent of tail abnormalities andedema in olive flounder were stronger than those of spotted bass in oilexposed groups. But the levels of CYP1A expression were lower thanthose of spotted bass. Our results confirm that the HSOS causedsignificant damage to resident fish embryos and different toxicsensitivity were investigated in both species at morphological andbiochemical levels. This study was supported by research fund from theKorean Ministry of Land, Transport and Maritime Affairs
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/26278
Bibliographic Citation
SETAC, pp.1, 2014
Publisher
SETAC
Type
Conference
Language
English
Publisher
SETAC
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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