6,6′-Bieckol protects insulinoma cells against high glucose-induced glucotoxicity by reducing oxidative stress and apoptosis
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Title
- 6,6′-Bieckol protects insulinoma cells against high glucose-induced glucotoxicity by reducing oxidative stress and apoptosis
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Author(s)
- Park, M.-H.; Heo, S.-J.; Kim, K.-N.; Ahn, G.; Park, P.-J.; Moon, S.-H.; Jeon, B.-T.; Lee, S.-H.
- KIOST Author(s)
- Heo, Soo Jin(허수진)
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Alternative Author(s)
- 허수진
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Publication Year
- 2015
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Abstract
- Pancreatic β cells are highly sensitive to oxidative stress, which might play an important role in β cell death in diabetes. The protective effect of 6,6′-bieckol, a phlorotannin polyphenol compound purified from Ecklonia cava, against high glucose-induced glucotoxicity was investigated in rat insulinoma cells. High glucose (30 mM) treatment induced the death of rat insulinoma cells, but treatment with 10 or 50 μg/mL 6,6′-bieckol significantly inhibited the high glucose-induced glucotoxicity. Furthermore, treatment with 6,6′-bieckol dose-dependently reduced the level of thiobarbituric acid reactive substances, generation of intracellular reactive oxygen species, and the level of nitric oxide, all of which were increased by high glucose concentration. In addition, 6,6′-bieckol protected rat insulinoma cells from apoptosis under high-glucose conditions. These effects were associated with increased expression of the anti-apoptotic protein Bcl-2 and reduced expression of the pro-apoptotic protein Bax. These findings indicate that 6,6′-bieckol could be used as a potential nutraceutical agent offering protection against the glucotoxicity caused by hyperglycemia-induced oxidative stress associated with diabetes. © 2015 Elsevier B.V.
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ISSN
- 0367-326X
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/2605
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DOI
- 10.1016/j.fitote.2015.08.014
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Bibliographic Citation
- Fitoterapia, v.106, pp.135 - 140, 2015
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Publisher
- Elsevier
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Subject
- 6,6' bioeckol; cytoprotective agent; glucose; nitric oxide; polyphenol derivative; protein Bax; protein bcl 2; reactive oxygen metabolite; thiobarbituric acid reactive substance; unclassified drug; 6,6' -bieckol; Bax protein, rat; dioxin; glucose; nitric oxide; protein Bax; protein bcl 2; reactive oxygen metabolite; thiobarbituric acid reactive substance; animal cell; apoptosis; Article; cell protection; cell viability; concentration response; controlled study; drug isolation; Ecklonia cava; glucotoxicity; insulinoma cell line; lipid peroxidation; nonhuman; oxidative stress; priority journal; protein expression; rat; tumor cell; animal; brown alga; cell survival; chemical structure; chemistry; drug effects; insulinoma; metabolism; oxidative stress; pancreas tumor; pathology; tumor cell line; Animals; Apoptosis; bcl-2-Associated X Protein; Cell Line, Tumor; Cell Survival; Dioxins; Glucose; Insulinoma; Lipid Peroxidation; Molecular Structure; Nitric Oxide; Oxidative Stress; Pancreatic Neoplasms; Phaeophyta; Proto-Oncogene Proteins c-bcl-2; Rats; Reactive Oxygen Species; Thiobarbituric Acid Reactive Substances
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Keywords
- 6,6′-bieckol; Apoptosis; High glucose; Oxidative stress; Rat insulinoma cells
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Type
- Article
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Language
- English
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Document Type
- Article
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