Immobilization of the hyperthermophilic archaeon Thermococcus onnurineus using amine-coated silica material for H2 production SCOPUS KCI

Title
Immobilization of the hyperthermophilic archaeon Thermococcus onnurineus using amine-coated silica material for H2 production
Author(s)
Bae, S.S.; Na, J.G.; Lee, S.-M.; Kang, S.G.; Lee, H.S.; Lee, J.-H.; Kim, T.W.
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)
Publication Year
2015
Abstract
Previously we reported that the hyperthermophilic archaeon, Thermococcus onnurineus NA1 is capable of producing hydrogen (H2) from formate, CO or starch. In this study, we describe the immobilization of T. onnurineus NA1 as an alternative means of H2 production. Amine-coated silica particles were effective in immobilizing T. onnurineus NA1 by electrostatic interaction, showing a maximum cell adsorption capacity of 71.7 mg-dried cells per g of particle. In three cycles of repeated-batch cultivation using sodium formate as the sole energy source, immobilized cells showed reproducible H2 production with a considerable increase in the initial production rate from 2.3 to 4.0 mmol I-1 h-1, mainly due to the increase in the immobilized cell concentration as the batch culture was repeated. Thus, the immobilized-cell system of T. onnurineus NA1 was demonstrated to be feasible for H2 production. This study is the first example of immobilized cells of hyperthermophilic archaea being used for the production of H2. © 2015, The Korean Society for Microbiology and Biotechnology.
ISSN
1598-642X
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2596
DOI
10.4014/mbl.1506.06003
Bibliographic Citation
Korean Journal of Microbiology and Biotechnology, v.43, no.3, pp.236 - 240, 2015
Publisher
Korean Society for Microbiolog and Biotechnology
Subject
amine; formic acid; hydrogen; starch; formic acid derivative; silicon dioxide; sodium formate; unclassified drug; adsorption; Article; bacterium culture; clinical effectiveness; concentration process; energy resource; hyperthermophilic archaeon; immobilized cell; nonhuman; particle size; static electricity; surface charge; Thermococcus onnurineus; immobilization; immobilized cell
Keywords
Amine-coated silica particle; Cell immobilization; Formate; H2 production; Thermococcus onnurineus NA1
Type
Article
Language
English
Document Type
Article
Publisher
Korean Society for Microbiolog and Biotechnology
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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