Phlorotannin protects oxidative stress through inhibiting expression of ROS and proinflammatory enzymes in high-glucose-induced HUVECs
DC Field | Value | Language |
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dc.contributor.author | 허수진 | - |
dc.contributor.author | 예보람 | - |
dc.contributor.author | 장지이 | - |
dc.contributor.author | 김민선 | - |
dc.contributor.author | 이승홍 | - |
dc.contributor.author | 전유진 | - |
dc.date.accessioned | 2020-07-16T02:53:51Z | - |
dc.date.available | 2020-07-16T02:53:51Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2014-10-27 | - |
dc.identifier.uri | https://sciwatch.kiost.ac.kr/handle/2020.kiost/25899 | - |
dc.description.abstract | Hyperglycemia-induced oxidative stress accelerates endothelial cell dysfunctions, which cause various complications of diabetes. The protective effects of 6,6′-bieckol (BEK), one of phlorotannin compound purified from Ecklonia cava against high-glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30 mM) treatment induced HUVECs’ cell death, but BEK, at concentration 10 or 50 μg/ml, significantly inhibited the high-glucose-induced cytotoxicity. Furthermore, treatment with BEK dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation, and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-kappa B (NF-κB) proteins in HUVECs, but BEK treatment reduced the overexpressions of these proteins. These findings indicate that BEK is a potential therapeutic agent that will prevent diabetic endothelial dysfunction and related complications. high-glucose-induced oxidative stress was investigated using human umbilical vein endothelial cells (HUVECs), which is susceptible to oxidative stress. High glucose (30 mM) treatment induced HUVECs’ cell death, but BEK, at concentration 10 or 50 μg/ml, significantly inhibited the high-glucose-induced cytotoxicity. Furthermore, treatment with BEK dose-dependently decreased thiobarbituric acid reactive substances (TBARS), intracellular reactive oxygen species (ROS) generation, and nitric oxide level increased by high glucose. In addition, high glucose levels induced the overexpressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and nuclear factor-kappa B (NF-κB) proteins in HUVECs, but BEK treatment reduced the overexpressions of these proteins. These findings indicate that BEK is a potential therapeutic agent that will prevent diabetic endothelial dysfunction and related complications. | - |
dc.description.uri | 2 | - |
dc.language | English | - |
dc.publisher | 한국해양바이오학회 | - |
dc.relation.isPartOf | The 10th KSMB Annual Meeting & Symposium | - |
dc.title | Phlorotannin protects oxidative stress through inhibiting expression of ROS and proinflammatory enzymes in high-glucose-induced HUVECs | - |
dc.type | Conference | - |
dc.citation.conferencePlace | KO | - |
dc.citation.endPage | 93 | - |
dc.citation.startPage | 93 | - |
dc.citation.title | The 10th KSMB Annual Meeting & Symposium | - |
dc.contributor.alternativeName | 허수진 | - |
dc.contributor.alternativeName | 예보람 | - |
dc.contributor.alternativeName | 장지이 | - |
dc.contributor.alternativeName | 김민선 | - |
dc.identifier.bibliographicCitation | The 10th KSMB Annual Meeting & Symposium, pp.93 | - |
dc.description.journalClass | 2 | - |