Rapid and sensitive detection of iridovirus by loop-mediated isothemal amplification (LAMP)

Title
Rapid and sensitive detection of iridovirus by loop-mediated isothemal amplification (LAMP)
Author(s)
황진익; 서승석; 박미례; 김종오; 오명주; 이석찬; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2014-11-06
Abstract
Red seabream iridovirus (RSIV), a member of the Iridoviridae family, is the causative pathogen for some of the most explosive epidemics of emerging viral diseases in many Asian countries, leading to huge economic losses in aquaculture. Rapid molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of RSIV infection. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of RSIV infection in fishes was developed. Using a set of synthesized primers matching a specific region of the RSIV genome (GenBank accession no.: AB666336.1), the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficiently detect RSIV infection in red sea bream. Our results provide a simple and convenient method for the detection of viral infection in aquatic organisms.d molecular detection for surveillance or diagnosis has been a critical component in reducing the prevalence of RSIV infection. In the present study, a novel and highly specific LAMP assay for the sensitive and rapid detection of RSIV infection in fishes was developed. Using a set of synthesized primers matching a specific region of the RSIV genome (GenBank accession no.: AB666336.1), the efficiency and specificity of the LAMP assay were optimized in terms of the reaction temperature and DNA polymerase concentration, as they are the main determinants of the sensitivity and specificity of the LAMP assay. In particular, we demonstrated that our assay could be applied to efficiently detect RSIV infection in red sea bream. Our results provide a simple and convenient method for the detection of viral infection in aquatic organisms.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25808
Bibliographic Citation
한국해양학회 추계학술대회 논문집, pp.144, 2014
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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