Loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus (NNV)

Title
Loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus (NNV)
Author(s)
황진익; 서승석; 박미례; 김종오; 오명주; 이석찬; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2014-11-06
Abstract
A loop-mediated isothermal amplification (LAMP) of DNA is currently one of the most commonly used molecular diagnostic tools due to its simple, fast and easy to amplify target DNA under isothermal conditions. In the present study, a LAMP test was designed from the coat protein gene of Nervous necrosis virus (NNV) which has been responsible for some of the most explosive epidemics of emerging viral diseases in many Asian countries, resulting in huge economical losses on a fish farm. Using a set of the synthesized primers matching the specific region of the NNV gene sequences provided by NCBI database, not originated from NNV-infected fish, we observed that the efficiency and specificity of LAMP were mainly dependent on the concentration of DNA polymerase and reaction temperature, suggesting that they act as main determinants in LAMP assay. In particular, we demonstrated that they were even applied to detect NNV infection in red sea bream. These data suggest that the development of LAMP primers based on genetic information provided by public database, not virus-infected samples, may provide very simple and convenient method to determine viral infection in aquatic organism.st was designed from the coat protein gene of Nervous necrosis virus (NNV) which has been responsible for some of the most explosive epidemics of emerging viral diseases in many Asian countries, resulting in huge economical losses on a fish farm. Using a set of the synthesized primers matching the specific region of the NNV gene sequences provided by NCBI database, not originated from NNV-infected fish, we observed that the efficiency and specificity of LAMP were mainly dependent on the concentration of DNA polymerase and reaction temperature, suggesting that they act as main determinants in LAMP assay. In particular, we demonstrated that they were even applied to detect NNV infection in red sea bream. These data suggest that the development of LAMP primers based on genetic information provided by public database, not virus-infected samples, may provide very simple and convenient method to determine viral infection in aquatic organism.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25806
Bibliographic Citation
한국해양학회 추계학술대회 논문집, pp.143, 2014
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse