EFFECTIVENESS OF MICROSATELLITE MARKERS FOR PARENTAGE ANALYSIS OF GIANT GROUPER (Epinephelus lanceolatus) BROODSTOCK

Title
EFFECTIVENESS OF MICROSATELLITE MARKERS FOR PARENTAGE ANALYSIS OF GIANT GROUPER (Epinephelus lanceolatus) BROODSTOCK
Author(s)
김근식; 노충환; 남윤권; Ahemad Sade; 방인철
KIOST Author(s)
Noh, Choong Hwan(노충환)
Publication Year
2015-04-17
Abstract
Giant grouper (Epinephelus lanceolatus) is an endangered species considered as a vulnerable grade-organism in the International Union for Conservation of Nature (IUCN) red list. As a fundamental baseline study for establishing a giant grouper broodstock management system, the efficiency for parentage analysis was evaluated by using microsatellite makers previously available in this species. The eight microsatellites generated a total 52 alleles from 32 individuals, the mean expected heterozygosity was 0.663, and mean inbreeding coefficient was 0.011, consequently suggesting that the present broodstock has retained genetic diversity. However, our analysis also recommended the collection of more broodfish for more stable brood line, since the estimated value of the effective population size was proven to be 35. The average probability of identity was 6.85×10-11. NE-2P and NE-PP of paternity non-exclusion probabilities were 0.00835 and 0.00027, respectively. As the result of principle coordinate analysis, the genotype of broodstock was not overlapped, suggesting that the management system of giant grouper based on eight selected microsatellite markers might be effective, although further validation with extended number of broodfish might also be needed in future. Data of present study could be a useful basis to avoid the unwanted selection of broodfish that possess close genetic relationship with current broodstock, aner broodstock management system, the efficiency for parentage analysis was evaluated by using microsatellite makers previously available in this species. The eight microsatellites generated a total 52 alleles from 32 individuals, the mean expected heterozygosity was 0.663, and mean inbreeding coefficient was 0.011, consequently suggesting that the present broodstock has retained genetic diversity. However, our analysis also recommended the collection of more broodfish for more stable brood line, since the estimated value of the effective population size was proven to be 35. The average probability of identity was 6.85×10-11. NE-2P and NE-PP of paternity non-exclusion probabilities were 0.00835 and 0.00027, respectively. As the result of principle coordinate analysis, the genotype of broodstock was not overlapped, suggesting that the management system of giant grouper based on eight selected microsatellite markers might be effective, although further validation with extended number of broodfish might also be needed in future. Data of present study could be a useful basis to avoid the unwanted selection of broodfish that possess close genetic relationship with current broodstock, an
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25638
Bibliographic Citation
(사)한국해양생명과학회 창립학술대회, pp.197, 2015
Publisher
(사)한국해양생명과학회
Type
Conference
Language
English
Publisher
(사)한국해양생명과학회
Related Researcher
Research Interests

Fish genetics and breeding,Living modified organism,Sbtropical mariculture,어류유전육종,어류형질전환,아열대어류양식

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse