PCR-based Assay for Detection of Five Coral Pathogens

Title
PCR-based Assay for Detection of Five Coral Pathogens
Author(s)
우선옥; 이택견
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2015-04-30
Abstract
Over the last 30 years, coral reef assessment has provided an extensive description of certain responses at population and community levels in terms of coral cover, diversity and population dynamics of other reef species. However, with only these descriptive approaches for assessment are incapable of identifying the causes of deterioration of coral reef ecosystems. Some microorganisms have been identified as pathogenic agents responsible for various outbreaks of coral disease. But most of diseases have been implicated within a subset of corals, leaving differential in our knowledge of the host range and geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in coral species using PCR-based assays. Total 4 pathogen-specific 16s rDNA primer sets and 1 Vibrio choleras Lux R gene primer set were designed for detecting coral pathogenic disease. The pathogenic bacteria used in this study are Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, and Vibrio carchariae. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan (Jeju Island -Sungsan, Munsum Japan - Wakayama , Kochi Taiwan - Green Island, Kenting). After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in corals. these descriptive approaches for assessment are incapable of identifying the causes of deterioration of coral reef ecosystems. Some microorganisms have been identified as pathogenic agents responsible for various outbreaks of coral disease. But most of diseases have been implicated within a subset of corals, leaving differential in our knowledge of the host range and geographic extent of a given pathogen. In this study, we purposed the rapid and inexpensive detection of pathogens in coral species using PCR-based assays. Total 4 pathogen-specific 16s rDNA primer sets and 1 Vibrio choleras Lux R gene primer set were designed for detecting coral pathogenic disease. The pathogenic bacteria used in this study are Aurantimonas coralicida, Serratia marcescens, Vibrio shilonii, Vibrio coralliilyticus, and Vibrio carchariae. The assay was applied to coral samples from the Jeju Island, Japan and Taiwan (Jeju Island -Sungsan, Munsum Japan - Wakayama , Kochi Taiwan - Green Island, Kenting). After the identification of infected samples, the PCR-based assay is to be evaluated for detection of presence of pathogenic bacteria in corals.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25595
Bibliographic Citation
한국미생물학회, pp.176, 2015
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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