Microphytoplankton community structure of a Cochlodinium polykrikoides bloom in the Tongyeong coastal waters of South Korea using morphological and 454 pyrosequencing

Title
Microphytoplankton community structure of a Cochlodinium polykrikoides bloom in the Tongyeong coastal waters of South Korea using morphological and 454 pyrosequencing
Author(s)
허진비; 정승원
KIOST Author(s)
Jung, Seung Won(정승원)
Publication Year
2015-05-22
Abstract
Massive phytoplankton blooms such as the annual Cochlodinium polykrikoides proliferation in the Tongyeong coastal waters (TCW) of South Korea have a significant influence on planktonic community structure and food web dynamics. Additionally, fish farms in the TCW are severely damaged by Harmful algal blooms, particularly C. polykrikoides. Although the ecology of the TCW is relatively complex, it has been reasonably well studied across two decades of monitoring, including morphological observations of its planktonic organisms. Our objective was to study the under-explored phytoplankton diversity using morphological and 454 pyrosequencing methods. During C. polykrikoides summer bloom, the phytoplankton biomass increased by threefold while its composition decreased in size. The operational taxonomic units (OTUs) in 454 pyrosequencing analysis decreased by half and corresponded with results of the morphological analysis. At the genus level, pyrosequencing revealed the diatoms Chaetoceros, Skeletonema, Thalassiosira, Ditylum, and Leptocylindrus, and the dinoflagellates Akashiwo, Alexandrium, and Cochlodinium, which were highest in abundance. Fluctuating monthly patterns of Skeletonema, Leptocylindrus, Akashiwo, and Cochlodinium in morphological and 454 pyrosequencing results correlated significantly. Although morphological data were more detailed for diatoms than for other groups, 454 pyrosequencing and morphological resu, fish farms in the TCW are severely damaged by Harmful algal blooms, particularly C. polykrikoides. Although the ecology of the TCW is relatively complex, it has been reasonably well studied across two decades of monitoring, including morphological observations of its planktonic organisms. Our objective was to study the under-explored phytoplankton diversity using morphological and 454 pyrosequencing methods. During C. polykrikoides summer bloom, the phytoplankton biomass increased by threefold while its composition decreased in size. The operational taxonomic units (OTUs) in 454 pyrosequencing analysis decreased by half and corresponded with results of the morphological analysis. At the genus level, pyrosequencing revealed the diatoms Chaetoceros, Skeletonema, Thalassiosira, Ditylum, and Leptocylindrus, and the dinoflagellates Akashiwo, Alexandrium, and Cochlodinium, which were highest in abundance. Fluctuating monthly patterns of Skeletonema, Leptocylindrus, Akashiwo, and Cochlodinium in morphological and 454 pyrosequencing results correlated significantly. Although morphological data were more detailed for diatoms than for other groups, 454 pyrosequencing and morphological resu
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25429
Bibliographic Citation
한국해양학회 춘계 학술대회, pp.280, 2015
Publisher
한국해양학회
Type
Conference
Language
English
Publisher
한국해양학회
Related Researcher
Research Interests

Phytoplatnkon taxonomy,Phytoplatnkon physiology,Phycosphere,식물플랑크톤 분류,식물플랑크톤 생리,식물플랑크톤, 바이러스, 박테리아 관계

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