INTERSPECIES DIFFERENCES IN DEVELOPMENTAL TOXICITY AND RNA-SEQ GENE EXPRESSION PROFILING ON TWO MARINE EMBRYONIC FISH EXPOSED TO IRANIAN HEAVY CRUDE OIL

Title
INTERSPECIES DIFFERENCES IN DEVELOPMENTAL TOXICITY AND RNA-SEQ GENE EXPRESSION PROFILING ON TWO MARINE EMBRYONIC FISH EXPOSED TO IRANIAN HEAVY CRUDE OIL
Alternative Title
INTERSPECIES DIFFERENCES IN DEVELOPMENTAL TOXICITY AND RNA-SEQ GENE EXPRESSION PROFILING ON TWO MARINE EMBRYONIC FISH EXPOSED TO IRANIAN HEAVY CRUDE OIL
Author(s)
정지현; 김하나; 심원준; John P. Incardona
KIOST Author(s)
Jung, Jee Hyun(정지현)Shim, Won Joon(심원준)
Publication Year
2015-05-26
Abstract
Interspecies difference of developmental toxicity of crude oil on embryonic fish allows the prediction of injury extent to a number of resident fish species in oil spill sites. This study clarifies the comparative developmental effects of Iranian Heavy crude oil (IHCO) on the differences of biouptake and toxic sensitivity between embryonic spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus). From 24 hrs after exposure to IHCO, several complex effects, including the pericardium edema, spinal curvature, developmental delay and tail fin malformation were observed in both of embryonic fish. The extent of edema, spinal curvature and developmental delay in olive flounder were stronger than those of spotted bass in oil exposed groups, whereas the levels of CYP1A expression were lower than those of spotted bass. Here, we utilized highthroughput RNA-seq to characterize the developmental toxic effects of the crude oil. The assembled contigs contained 16,942 known unigenes in flounder and 167,049 known unigenes in spotted seabass in this study. Comparison of different gene expression between exposed to crude oil and control samples revealed that the number of 97~108 genes defferentially up-regulated but 448~419 genes differentially down-regulated at 4 hrs following exposure. Gene pathway analysis of the differentially expressed gene set indicated the immune and embryonic heart tube development, cellranian Heavy crude oil (IHCO) on the differences of biouptake and toxic sensitivity between embryonic spotted bass (Lateolabrax maculates) and olive flounder (Paralichthys olivaceus). From 24 hrs after exposure to IHCO, several complex effects, including the pericardium edema, spinal curvature, developmental delay and tail fin malformation were observed in both of embryonic fish. The extent of edema, spinal curvature and developmental delay in olive flounder were stronger than those of spotted bass in oil exposed groups, whereas the levels of CYP1A expression were lower than those of spotted bass. Here, we utilized highthroughput RNA-seq to characterize the developmental toxic effects of the crude oil. The assembled contigs contained 16,942 known unigenes in flounder and 167,049 known unigenes in spotted seabass in this study. Comparison of different gene expression between exposed to crude oil and control samples revealed that the number of 97~108 genes defferentially up-regulated but 448~419 genes differentially down-regulated at 4 hrs following exposure. Gene pathway analysis of the differentially expressed gene set indicated the immune and embryonic heart tube development, cell
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25408
Bibliographic Citation
International Symposium Pollutant Responses in Marine Organism (PRIMO), pp.194, 2015
Publisher
18th International Symposium Pollutant Responses in Marine Organism
Type
Conference
Language
English
Publisher
18th International Symposium Pollutant Responses in Marine Organism
Related Researcher
Research Interests

Microplastic pollution,Persistent Organic Pollutants,Oil Pollution,미세플라스틱 오염,잔류성 유기오염물질,유류오염

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