EFFECTS OF CRYOPRESERVATION ON 4 OF THE SERRANIDAE SPECIES AND INDUCING HYBRIDIZATION

Title
EFFECTS OF CRYOPRESERVATION ON 4 OF THE SERRANIDAE SPECIES AND INDUCING HYBRIDIZATION
Author(s)
방인철; 노충환; 강성원; 정태혁; 임한규
KIOST Author(s)
Noh, Choong Hwan(노충환)
Publication Year
2015-05-29
Abstract
It was observed that the red grouper sperm exhibited the highest post-thaw mobility with 10% glycerol as cryoprotectant while the giant grouper sperm showed the highest level of mobility with DMSO. For species of tiger grouper and hybrid grouper, glycerol was the most effective for the post-thaw mobility. Fertilization capacity of the cryopreserved sperm was evaluated in comparison with the natural red grouper sperm (control). Fertilization rate of the natural red grouper spermto the natural red grouper eggs was 89% with 31.3% of the fertilized eggs hatched. Meanwhile, the cryopreserved giant grouper sperm exhibited 29.9% fertilization rate with 16.0% hatch rate, the cryopreserved tiger grouper sperm showed 27.8% fertilization rate with 18.9% hatch rate and 9.1% of the cryopreserved hybrid grouper sperm was fertilized to the eggs with 0% hatched.ouper, glycerol was the most effective for the post-thaw mobility. Fertilization capacity of the cryopreserved sperm was evaluated in comparison with the natural red grouper sperm (control). Fertilization rate of the natural red grouper spermto the natural red grouper eggs was 89% with 31.3% of the fertilized eggs hatched. Meanwhile, the cryopreserved giant grouper sperm exhibited 29.9% fertilization rate with 16.0% hatch rate, the cryopreserved tiger grouper sperm showed 27.8% fertilization rate with 18.9% hatch rate and 9.1% of the cryopreserved hybrid grouper sperm was fertilized to the eggs with 0% hatched.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25400
Bibliographic Citation
World Aquaculture 2015 Jeju Meeting, pp.33, 2015
Publisher
World Aquaculture Society
Type
Conference
Language
English
Publisher
World Aquaculture Society
Related Researcher
Research Interests

Fish genetics and breeding,Living modified organism,Sbtropical mariculture,어류유전육종,어류형질전환,아열대어류양식

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