CHROMENE INHIBITS LPS-STIMULATED INFLAMMATORY MEDIATORS VIA NF-κB AND MAPKS PATHWAYS IN MACROPHAGES
-
Title
- CHROMENE INHIBITS LPS-STIMULATED INFLAMMATORY MEDIATORS VIA NF-κB AND MAPKS PATHWAYS IN MACROPHAGES
-
Author(s)
- 허수진; 오철홍; 강도형
- KIOST Author(s)
- Heo, Soo Jin(허수진); Oh, Chul Hong(오철홍); Kang, Do Hyung(강도형)
-
Alternative Author(s)
- 허수진; 오철홍; 강도형
-
Publication Year
- 2015-06-15
-
Abstract
- The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages. lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages.
-
URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/25386
-
Bibliographic Citation
- World Aquaculture 2015, pp.206, 2015
-
Publisher
- World Aquaculture
-
Type
- Conference
-
Language
- English
- Files in This Item:
-
There are no files associated with this item.
Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.