CHROMENE INHIBITS LPS-STIMULATED INFLAMMATORY MEDIATORS VIA NF-κB AND MAPKS PATHWAYS IN MACROPHAGES

Title
CHROMENE INHIBITS LPS-STIMULATED INFLAMMATORY MEDIATORS VIA NF-κB AND MAPKS PATHWAYS IN MACROPHAGES
Author(s)
허수진; 오철홍; 강도형
KIOST Author(s)
Heo, Soo Jin(허수진)Oh, Chulhong(오철홍)Kang, Do-Hyung(강도형)
Publication Year
2015-06-15
Abstract
The aim of this study was to identify a natural effective remedy for inflammation. We isolated a functional algal chromene compound from Sargassum siliquastrum, named sargachromanol D (SD). We evaluated the anti-inflammatory effect of SD on lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages. lipopolysaccharide (LPS)-exposed RAW 264.7 cells by measuring cell viability, cytotoxicity, and production of inflammatory mediators such as nitric oxide (NO), prostaglandin E2 (PGE2), inducible NO synthase (iNOS), cyclooxygenase-2 (COX-2), and the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α), interleukin (IL-1β, and IL-6). SD inhibited production of NO and PGE2 from LPS-induced cells by preventing the expression of inflammatory mediators such as iNOS and COX-2 in a dose-dependent manner. Concurrently, levels of the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6 were reduced with increasing concentrations of SD. In addition, SD inhibited the activation of NF-κB and mitogen-activated protein kinases (MAPKs) pathways in a concentration-dependent manner. These results indicate that SD inhibits LPS-stimulated inflammation by inhibition of the NF-κB and MAPKs pathways in macrophages.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25386
Bibliographic Citation
World Aquaculture 2015, pp.206, 2015
Publisher
World Aquaculture
Type
Conference
Language
English
Publisher
World Aquaculture
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