Microphytoplankton community structure of a Cochlodinium polykrikoides bloom in the Tongyeong coastal waters of South Korea using morphological and 454 pyrosequencing methods

Title
Microphytoplankton community structure of a Cochlodinium polykrikoides bloom in the Tongyeong coastal waters of South Korea using morphological and 454 pyrosequencing methods
Author(s)
정승원; 김현정; 임동일; 이택견
KIOST Author(s)
Jung, Seung Won(정승원)Kim, Hyun Jung(김현정)Lim, Dhongil(임동일)Lee, Taek Kyun(이택견)
Publication Year
2015-10-20
Abstract
Massive phytoplankton blooms such as the annual Cochlodinium polykrikoides proliferation in the Tongyeong coastal waters (TCW) of South Korea have a significant influence on planktonic community structure and food web dynamics. Additionally, fish farms in the TCW are severely damaged by harmful algal blooms, particularly C.polykrikoides. Although the ecology of the TCW is relatively complex, it has been reasonably well studied across two decades of monitoring, including morphological observations of its planktonic organisms. Our objective was to study the under-explored phytoplankton diversity using morphological and 454 pyrosequencing methods.During C. polykrikoides summer bloom, the phytoplankton biomass increased by threefold while its compositiondecreased in size. The operational taxonomic units (OTUs) in 454 pyrosequencing analysis decreased by half and corresponded with results of the morphological analysis. At the genus level, pyrosequencing revealed the diatoms Chaetoceros, Skeletonema, Thalassiosira, Ditylum, and Leptocylindrus, and the dinoflagellates Akashiwo, Alexandrium, and Cochlodinium, which were highest in abundance. Fluctuating monthly patterns of Skeletonema, Leptocylindrus, Akashiwo, and Cochlodinium in morphological and 454 pyrosequencing results correlated significantly. Although morphological data were more detailed for diatoms than for other groups, 454 pyrosequencing and morphological resul, fish farms in the TCW are severely damaged by harmful algal blooms, particularly C.polykrikoides. Although the ecology of the TCW is relatively complex, it has been reasonably well studied across two decades of monitoring, including morphological observations of its planktonic organisms. Our objective was to study the under-explored phytoplankton diversity using morphological and 454 pyrosequencing methods.During C. polykrikoides summer bloom, the phytoplankton biomass increased by threefold while its compositiondecreased in size. The operational taxonomic units (OTUs) in 454 pyrosequencing analysis decreased by half and corresponded with results of the morphological analysis. At the genus level, pyrosequencing revealed the diatoms Chaetoceros, Skeletonema, Thalassiosira, Ditylum, and Leptocylindrus, and the dinoflagellates Akashiwo, Alexandrium, and Cochlodinium, which were highest in abundance. Fluctuating monthly patterns of Skeletonema, Leptocylindrus, Akashiwo, and Cochlodinium in morphological and 454 pyrosequencing results correlated significantly. Although morphological data were more detailed for diatoms than for other groups, 454 pyrosequencing and morphological resul
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25246
Bibliographic Citation
PICES-2015, pp.238, 2015
Publisher
PICES-2015
Type
Conference
Language
English
Publisher
PICES-2015
Related Researcher
Research Interests

Phytoplatnkon taxonomy,Phytoplatnkon physiology,Phycosphere,식물플랑크톤 분류,식물플랑크톤 생리,식물플랑크톤, 바이러스, 박테리아 관계

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse