Development of Lactobacillus paracasei harboring nucleic acid-hydrolyzing 3D8 scFv as a preventive probiotic against murine norovirus infection SCIE SCOPUS

Cited 23 time in WEB OF SCIENCE Cited 27 time in Scopus
Title
Development of Lactobacillus paracasei harboring nucleic acid-hydrolyzing 3D8 scFv as a preventive probiotic against murine norovirus infection
Author(s)
Phuong Mai Hoang; Cho, Seungchan; Kim, Kee Eun; Byun, Sung June; Lee, Taek-Kyun; Lee, Sukchan
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
2015-03
Abstract
The protein 3D8 single-chain variable fragment (3D8 scFv) has potential anti-viral activity due to its ability to penetrate into cells and hydrolyze nucleic acids. Probiotic Lactobacillus paracasei engineered to secrete 3D8 scFv for oral administration was used to test the anti-viral effects of 3D8 scFv against gastrointestinal virus infections. We found that injection of 3D8 scFv into the intestinal lumen resulted in the penetration of 3D8 scFv into the intestinal villi and lamina propria. 3D8 scFv secreted from engineered L. paracasei retained its cell-penetrating and nucleic acid-hydrolyzing activities, which were previously shown with 3D8 scFv expressed in Escherichia coli. Pretreatment of RAW264.7 cells with 3D8 scFv purified from L. paracasei prevented apoptosis induction by murine norovirus infection and decreased messenger RNA (mRNA) expression of the viral capsid protein VP1. In a mouse model, oral administration of the engineered L. paracasei prior to murine norovirus infection reduced the expression level of mRNA encoding viral polymerase. Taken together, these results suggest that L. paracasei secreting 3D8 scFv provides a basis for the development of ingestible anti-viral probiotics active against gastrointestinal viral infection.
ISSN
0175-7598
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2524
DOI
10.1007/s00253-014-6257-7
Bibliographic Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.99, no.6, pp.2793 - 2803, 2015
Publisher
SPRINGER
Subject
MURINE-NOROVIRUS-1 INFECTION; INNATE IMMUNITY; LIGHT-CHAIN; BACTERIA; DELIVERY; CELLS; MICE; PEPTIDE; CASEI; GUT
Keywords
3D8 scFv; Anti-viral; Lactobacillus paracasei; Oral administration; Murine norovirus
Type
Article
Language
English
Document Type
Article
Publisher
SPRINGER
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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