One-Step and Quick Diagnostic Methods for Ballast Water Control

Title
One-Step and Quick Diagnostic Methods for Ballast Water Control
Author(s)
염승식
KIOST Author(s)
Yum, Seungshic(염승식)
Publication Year
2015-11-04
Abstract
Developing the convenient methods for microalgal (10-50 μm in size) detection, and judgement of the organisms’ dead or alive in treated ballast water has become a hot issue these days. Conventional microscopic method for these purposes is often laborious, tedious, and time-consuming. It also requires well-trained and disciplined zoo- and phyto-plankton investigators. Thus, easy, quick, accurate and efficient methods which could be utilized by ordinary, untrained people, and/or non-scientists, are necessary to overcome these difficulties. For these reasons, first, we have developed the immunochromatography based one-step kits for microalgae detection using monoclonal antibodies raised against particular proteins of microalgal species. The kits were proved to be efficient in microalgal detection. Second, we have found that the cell viability assay would be the powerful candidate for dead or alive judgement. Thus, these two tools could be applicable to the judgment of D2 standard which specifies the levels of viable organisms left in treated ballast water. often laborious, tedious, and time-consuming. It also requires well-trained and disciplined zoo- and phyto-plankton investigators. Thus, easy, quick, accurate and efficient methods which could be utilized by ordinary, untrained people, and/or non-scientists, are necessary to overcome these difficulties. For these reasons, first, we have developed the immunochromatography based one-step kits for microalgae detection using monoclonal antibodies raised against particular proteins of microalgal species. The kits were proved to be efficient in microalgal detection. Second, we have found that the cell viability assay would be the powerful candidate for dead or alive judgement. Thus, these two tools could be applicable to the judgment of D2 standard which specifies the levels of viable organisms left in treated ballast water.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25176
Bibliographic Citation
International Forum on BWM Technical Cooperation 2015, pp.20, 2015
Publisher
해양수산부
Type
Conference
Language
English
Publisher
해양수산부
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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