Metabolic collaboration of thermophlic microorganisms, evidence by metabolic and genomic analysis

Title
Metabolic collaboration of thermophlic microorganisms, evidence by metabolic and genomic analysis
Author(s)
권개경; 김경모; 이상헌; 양성현; 오지혜; 이정현
KIOST Author(s)
Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)
Publication Year
2015-11-05
Abstract
Total 12 strains affiliated with three genera were isolated from enriched culture broth with damaged algae at 50OC. Amongst only 1 strain affiliated with Microbulbifer thermotolerans MEBiC11484 could degrade kelp flakes into powder form, however, only small amounts of sugar was detected. When assayed with DNS method, strain MEBiC11484 could utilize laminarin, cellobiose, and xylan but the rate of cellobiose degradation was very low. Caenispirillum salinarum MEBiC11482, an another isolate, utilize cellobiose and laminarin very well. Xylanase activity was boosted when strains were mixed. Two set of alginate lyase, beta-agarase subunit a and b, a chitinase, beta-glucosidase, and endo-beta-xylanase genes were pedicted from genome of strain MEBiC11484. However, strain 11482 possess different kinds of glycosyl hydrolases. Genomic analysis showed that starch and glycerophospholipid metabolc pathways were completed by combination of 3 strains.wever, only small amounts of sugar was detected. When assayed with DNS method, strain MEBiC11484 could utilize laminarin, cellobiose, and xylan but the rate of cellobiose degradation was very low. Caenispirillum salinarum MEBiC11482, an another isolate, utilize cellobiose and laminarin very well. Xylanase activity was boosted when strains were mixed. Two set of alginate lyase, beta-agarase subunit a and b, a chitinase, beta-glucosidase, and endo-beta-xylanase genes were pedicted from genome of strain MEBiC11484. However, strain 11482 possess different kinds of glycosyl hydrolases. Genomic analysis showed that starch and glycerophospholipid metabolc pathways were completed by combination of 3 strains.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25169
Bibliographic Citation
2015 한국미생물학회연합 국제학술대회, pp.76, 2015
Publisher
한국미생물학회연합
Type
Conference
Language
English
Publisher
한국미생물학회연합
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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