Metabolic collaboration of thermophlic microorganisms, evidence by metabolic and genomic analysis

Abstract

Total 12 strains affiliated with three genera were isolated from enriched culture broth with damaged algae at 50OC. Amongst only 1 strain affiliated with Microbulbifer thermotolerans MEBiC11484 could degrade kelp flakes into powder form, however, only small amounts of sugar was detected. When assayed with DNS method, strain MEBiC11484 could utilize laminarin, cellobiose, and xylan but the rate of cellobiose degradation was very low. Caenispirillum salinarum MEBiC11482, an another isolate, utilize cellobiose and laminarin very well. Xylanase activity was boosted when strains were mixed. Two set of alginate lyase, beta-agarase subunit a and b, a chitinase, beta-glucosidase, and endo-beta-xylanase genes were pedicted from genome of strain MEBiC11484. However, strain 11482 possess different kinds of glycosyl hydrolases. Genomic analysis showed that starch and glycerophospholipid metabolc pathways were completed by combination of 3 strains.wever, only small amounts of sugar was detected. When assayed with DNS method, strain MEBiC11484 could utilize laminarin, cellobiose, and xylan but the rate of cellobiose degradation was very low. Caenispirillum salinarum MEBiC11482, an another isolate, utilize cellobiose and laminarin very well. Xylanase activity was boosted when strains were mixed. Two set of alginate lyase, beta-agarase subunit a and b, a chitinase, beta-glucosidase, and endo-beta-xylanase genes were pedicted from genome of strain MEBiC11484. However, strain 11482 possess different kinds of glycosyl hydrolases. Genomic analysis showed that starch and glycerophospholipid metabolc pathways were completed by combination of 3 strains.