2-Bromo-5-Hydroxy-4-Methoxybenzaldehyde Suppresses the Activation of Inflammatory Mediators in LPS-Stimulated RAW 264.7 Cells

Title
2-Bromo-5-Hydroxy-4-Methoxybenzaldehyde Suppresses the Activation of Inflammatory Mediators in LPS-Stimulated RAW 264.7 Cells
Author(s)
허수진; 예보람; 김민선; 김준성
KIOST Author(s)
Heo, Soo Jin(허수진)Kim, Junseong(김준성)
Publication Year
2015-11-10
Abstract
In this study, we nvestigated the aility of 2-bromo-5-hydroxy-4-methoxybenzaldehyde (BMH) to inhibit lipopolysaccharide (LPS)-induced expressin of pro-inflammatory mediators in mouse macrophage (RAW 264.7) cells, and the molecular mechanism through which this inhibition occurred. Our results indicated that BHM downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglanclin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, BHM suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TN F)-α. In addition, BHM inhibited the activation of NF-кB and mitogen-activated protein kianses (MAPKs) pathways in a concentration-dependent manner. These results indicate that BHM inhibits LPS-stimuated inflammation by inhibition of the NF-кB and MAPKs pathways in macrophages. through which this inhibition occurred. Our results indicated that BHM downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglanclin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, BHM suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TN F)-α. In addition, BHM inhibited the activation of NF-кB and mitogen-activated protein kianses (MAPKs) pathways in a concentration-dependent manner. These results indicate that BHM inhibits LPS-stimuated inflammation by inhibition of the NF-кB and MAPKs pathways in macrophages.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25080
Bibliographic Citation
2015 KFN International Symposium and Annual Meeting, pp.363, 2015
Publisher
The Korean Society of Food Science and Nutrition
Type
Conference
Language
English
Publisher
The Korean Society of Food Science and Nutrition
Related Researcher
Research Interests

Marine Life Science,Marine Natural Products,Bioactivities,해양생명과학,해양천연물학,생리활성

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