2-Bromo-5-Hydroxy-4-Methoxybenzaldehyde Suppresses the Activation of Inflammatory Mediators in LPS-Stimulated RAW 264.7 Cells
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Title
- 2-Bromo-5-Hydroxy-4-Methoxybenzaldehyde Suppresses the Activation of Inflammatory Mediators in LPS-Stimulated RAW 264.7 Cells
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Author(s)
- 허수진; 예보람; 김민선; 김준성
- KIOST Author(s)
- Heo, Soo Jin(허수진); Kim, Jun Seong(김준성)
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Alternative Author(s)
- 허수진; 예보람; 김민선; 김준성
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Publication Year
- 2015-11-10
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Abstract
- In this study, we nvestigated the aility of 2-bromo-5-hydroxy-4-methoxybenzaldehyde (BMH) to inhibit lipopolysaccharide (LPS)-induced expressin of pro-inflammatory mediators in mouse macrophage (RAW 264.7) cells, and the molecular mechanism through which this inhibition occurred. Our results indicated that BHM downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglanclin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, BHM suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TN F)-α. In addition, BHM inhibited the activation of NF-кB and mitogen-activated protein kianses (MAPKs) pathways in a concentration-dependent manner. These results indicate that BHM inhibits LPS-stimuated inflammation by inhibition of the NF-кB and MAPKs pathways in macrophages. through which this inhibition occurred. Our results indicated that BHM downregulated LPS-induced nitric oxide (NO) synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, thereby reducing the production of NO and prostaglanclin E2 (PGE2) in LPS-activated RAW 264.7 cells. Furthermore, BHM suppressed LPS-induced production of pro-inflammatory cytokines such as interleukin (IL)-1β, IL-6, and tumor necrosis factor (TN F)-α. In addition, BHM inhibited the activation of NF-кB and mitogen-activated protein kianses (MAPKs) pathways in a concentration-dependent manner. These results indicate that BHM inhibits LPS-stimuated inflammation by inhibition of the NF-кB and MAPKs pathways in macrophages.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/25080
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Bibliographic Citation
- 2015 KFN International Symposium and Annual Meeting, pp.363, 2015
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Publisher
- The Korean Society of Food Science and Nutrition
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Type
- Conference
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Language
- English
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