Endocrine disruption effects in cadmium exposed marine medaka fish (Oryzias javanicus)

Title
Endocrine disruption effects in cadmium exposed marine medaka fish (Oryzias javanicus)
Author(s)
이다영; 우선옥; 염승식
KIOST Author(s)
Lee, Na Young(이나영)Woo, Seon Ock(우선옥)Yum, Seung Shic(염승식)
Alternative Author(s)
이나영; 우선옥; 염승식
Publication Year
2015-11-13
Abstract
Transcripts response induced by acute toxicity of cadmium (Cd) in marine medaka was evaluated. The median lethal concentration of the animals (LC50) was determined to be 27.8 mg/L after exposure to Cd for 48 h. Differential gene expression profiling of the marine medaka was performed with a cDNA microarray after exposure to 300 µ g/L of Cd (about 1/100 of 48-h LC50) for 48 h to understand the acute toxicity of Cd. We identified 774 differentially expressed genes the expressions of 440 genes were up-regulated and those of 334 genes were down-regulated over 1.5-fold (P < 0.05). The up-regulated and down-regulated genes were assembled into 24 and 22 functional groups, respectively, based on the eukaryotic orthologous groups (KOG) classification. The differentially expressed gene profile of Cd exposed fish was compared with that of bisphenol A (BPA) and 17β-estradiol (E2). Thirty-two genes were common in three experimental groups, and these genes could be categorized to carcinogenesis, detoxification, endocrine disruption, immune, inorganic ion transport, lipid metabolism, membrane signal receptor, spermatogenesis, transcriptional factor, transmembrane glycoprotein receptor, and other biological responses. The results showed that Cd could induce the endocrine disruption in fish species.profiling of the marine medaka was performed with a cDNA microarray after exposure to 300 µ g/L of Cd (about 1/100 of 48-h LC50) for 48 h to understand the acute toxicity of Cd. We identified 774 differentially expressed genes the expressions of 440 genes were up-regulated and those of 334 genes were down-regulated over 1.5-fold (P < 0.05). The up-regulated and down-regulated genes were assembled into 24 and 22 functional groups, respectively, based on the eukaryotic orthologous groups (KOG) classification. The differentially expressed gene profile of Cd exposed fish was compared with that of bisphenol A (BPA) and 17β-estradiol (E2). Thirty-two genes were common in three experimental groups, and these genes could be categorized to carcinogenesis, detoxification, endocrine disruption, immune, inorganic ion transport, lipid metabolism, membrane signal receptor, spermatogenesis, transcriptional factor, transmembrane glycoprotein receptor, and other biological responses. The results showed that Cd could induce the endocrine disruption in fish species.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/25057
Bibliographic Citation
대한독성유전단백체학회, pp.154, 2015
Publisher
대한독성유전단백체학회
Type
Conference
Language
English
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