Saline Wastewater Treatment from Land Fish-Farm by Biological Sequencing Batch Reactor (Sbr) by High Efficiency Nitrogen and Phosphorus Removing Bacteria

Abstract

Research has been rarely reported to develop saline wastewater from land fish-farm. Unfortunately, several systems require very high costs to operate and treat saline wastewater from land fish-farm and coastal area. Thus, there are urgent requirements to develop the low cost and eco-friendly systems for land fish farms. This study was conducted to apply eco-friendly sediment with high efficient bacteria to lab-scale biological SBR system for saline wastewater from land fish-farms. Nitrogen and phosphorus high removal efficient bacteria were screened of 960 strains isolated from seawater and sediment. Nitrogen removal high efficient bacteria Bacillus aryabhattai strain KGN1 and phosphorus removal high efficient bacteria Vibrio neocaledonicus strain KGP1 were designed as 86.0% nitrogen removal efficiency and 99.9% phosphorus removal efficiency in 10 h, respectively. High efficient bacteria were applied to marine sediment as eco-friendly material, and adapted to make eco-friendly sludge. The ecofriendly sludge was applied to lab-scale SBR system and operated for 7 months with various operating 7 conditions. Lab-scale biological SBR system showed nitrogen and phosphorus removal efficiency with 87.0% and 76.6%, respectively in optimal operating condition (3h/cylcle): aeration for 1 hour, settle for 1 hour and idle for 1 hour at the total volume of 40 L. The optimal kinetic parameters were 0.33 of food-to-mass ratio (F/M equirements to develop the low cost and eco-friendly systems for land fish farms. This study was conducted to apply eco-friendly sediment with high efficient bacteria to lab-scale biological SBR system for saline wastewater from land fish-farms. Nitrogen and phosphorus high removal efficient bacteria were screened of 960 strains isolated from seawater and sediment. Nitrogen removal high efficient bacteria Bacillus aryabhattai strain KGN1 and phosphorus removal high efficient bacteria Vibrio neocaledonicus strain KGP1 were designed as 86.0% nitrogen removal efficiency and 99.9% phosphorus removal efficiency in 10 h, respectively. High efficient bacteria were applied to marine sediment as eco-friendly material, and adapted to make eco-friendly sludge. The ecofriendly sludge was applied to lab-scale SBR system and operated for 7 months with various operating 7 conditions. Lab-scale biological SBR system showed nitrogen and phosphorus removal efficiency with 87.0% and 76.6%, respectively in optimal operating condition (3h/cylcle): aeration for 1 hour, settle for 1 hour and idle for 1 hour at the total volume of 40 L. The optimal kinetic parameters were 0.33 of food-to-mass ratio (F/M ratio), 0.41 of cell yield coefficient (Y), 0.64 of specific growth coefficient (μ) and 0.63 of sludge volume index (SVI), respectively. In addition, NGS (next generation sequencing) and qPCR analysis showed the abundance of Vibrio neocaledonicus strain KGP1 was maintained and adapted up to 32% of total microbial community of activated sludge in the lab-scale SBR reactor. From the study, our findings will give possible solution to develop biological treatment of saline wastewater fromland fish-farm eco-friendly.