Target tissues of the manila clam Ruditapes philippinarum for studying metal accumulation and biomarkers in environmental monitoring

Title
Target tissues of the manila clam Ruditapes philippinarum for studying metal accumulation and biomarkers in environmental monitoring
Author(s)
원은지; 김경태; 최진영; 김은수; 나공태
KIOST Author(s)
Kim, Kyung Tae(김경태)Choi, Jin Young(최진영)Ra, Kongtae(나공태)
Publication Year
2016-06-21
Abstract
To characterize the target tissues of the manila clam Ruditapes philippinarum for use as test organisms for environmental study, the accumulation of trace metals and three biomarkers were measured in different organs. Exposure with Cu and Pb carried out under laboratory conditions revealed a linear uptake of metals throughout the experimental period in each tissue. In particular, significant increase was observed in gills and mantle. The increase of intracellular reactive oxygen species showed the great potential of gills as a target tissue for both Cu and Pb exposure. The highest activity of glutathione-S-transferase and their relative increase in activity were also observed in gills. Metallothionein-like protein levels, however, increased greatly in the digestive gland and mantle during Cu and Pb exposure, respectively, although all tissues, except the foot, showed significant changes after 24 h of metal exposure. In the field study, the highest concentration of metals was recorded in the gills and mantle, accounting for over 50% of the total accumulated metal in all sites. Additionally, Cu and Pb increased significantly in these two organs, respectively. However, the order of accumulation rate in laboratory exposure was not concomitant with those of the lab-based study, suggesting that different routes of metal uptake and exposure duration induce distinct partitioning of metals and regulating system in R. philippib carried out under laboratory conditions revealed a linear uptake of metals throughout the experimental period in each tissue. In particular, significant increase was observed in gills and mantle. The increase of intracellular reactive oxygen species showed the great potential of gills as a target tissue for both Cu and Pb exposure. The highest activity of glutathione-S-transferase and their relative increase in activity were also observed in gills. Metallothionein-like protein levels, however, increased greatly in the digestive gland and mantle during Cu and Pb exposure, respectively, although all tissues, except the foot, showed significant changes after 24 h of metal exposure. In the field study, the highest concentration of metals was recorded in the gills and mantle, accounting for over 50% of the total accumulated metal in all sites. Additionally, Cu and Pb increased significantly in these two organs, respectively. However, the order of accumulation rate in laboratory exposure was not concomitant with those of the lab-based study, suggesting that different routes of metal uptake and exposure duration induce distinct partitioning of metals and regulating system in clams.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24701
Bibliographic Citation
8th International Conference on Marine Pollution and Ecotoxicology, pp.P53, 2016
Publisher
The University of Hong Kong, City University of Hong Kong
Type
Conference
Language
English
Publisher
The University of Hong Kong, City University of Hong Kong
Related Researcher
Research Interests

Management of marine environment,Marine pollution,Trace metal(Heavy metal),해양환경관리,해양오염,미량금속(중금속)

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