Echinicola donghaensis sp. nov., a mesophilic marine bacterium isolated from a sea urchin (Strongylocentrotus intermedius) in the East Sea of Korea

Title
Echinicola donghaensis sp. nov., a mesophilic marine bacterium isolated from a sea urchin (Strongylocentrotus intermedius) in the East Sea of Korea
Author(s)
정유정; 양성현; 권개경; 배승섭
KIOST Author(s)
Kwon, Kae Kyoung(권개경)
Alternative Author(s)
양성현; 권개경
Publication Year
2016-08-22
Abstract
A yellow-orange pigmented marine bacterium, designated MEBiC08714 was isolated from the sea urchin Strongylocentrotus intermedius in the East Sea of Korea. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that strain MEBiC08714T belonged to genus Echinicola and showed that this strain was most closely related to Echinicola vietnamensisT (97.02 %), Echinicola shivajiensisT (96.04 %), Echinicola jeungdonensisT (96.17 %) and Echinicola pacificaT (95.01 %). Cells were strictly aerobic, Gram– negative rods, flexirubin pigment-negative and motile by gliding. Growth was observed at 20-35 °C (optimum 25 °C), at pH 6-11 (optimum pH 7.0) and with 0-13 % NaCl (optimum 2 %). This strain was able to hydrolyse agar and starch. The major fatty acids were iso-C15:0 (27.5 %) iso-C17:0 3-OH (11.5 %), anteiso-C15:0 (5.2 %), C17:1ω6c (4.7 %) and summed feature 3 (20.3 %, C16:1ω7c and/or C16:1ω6c). The major respiratory quinone was MK-7. On the basis of these polyphasic taxonomic data in this study, strain MEBiC08714T represents a novel species of the genus Echinicola, for which the name Echinicola donghaensis sp. nov. is proposed. The type strain is MEBiC08714T (=KCTC 52050T).iC08714T belonged to genus Echinicola and showed that this strain was most closely related to Echinicola vietnamensisT (97.02 %), Echinicola shivajiensisT (96.04 %), Echinicola jeungdonensisT (96.17 %) and Echinicola pacificaT (95.01 %). Cells were strictly aerobic, Gram– negative rods, flexirubin pigment-negative and motile by gliding. Growth was observed at 20-35 °C (optimum 25 °C), at pH 6-11 (optimum pH 7.0) and with 0-13 % NaCl (optimum 2 %). This strain was able to hydrolyse agar and starch. The major fatty acids were iso-C15:0 (27.5 %) iso-C17:0 3-OH (11.5 %), anteiso-C15:0 (5.2 %), C17:1ω6c (4.7 %) and summed feature 3 (20.3 %, C16:1ω7c and/or C16:1ω6c). The major respiratory quinone was MK-7. On the basis of these polyphasic taxonomic data in this study, strain MEBiC08714T represents a novel species of the genus Echinicola, for which the name Echinicola donghaensis sp. nov. is proposed. The type strain is MEBiC08714T (=KCTC 52050T).
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24640
Bibliographic Citation
ISME, pp.14, 2016
Publisher
ISEM
Type
Conference
Language
English
Publisher
ISEM
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