Rapid Diagnosis of Red Sea Bream Iridovirus Infection using Recombinant Single-chain Variable Fragment (scFv)

Rapid Diagnosis of Red Sea Bream Iridovirus Infection using Recombinant Single-chain Variable Fragment (scFv)
박동훈; 서한열; 이용준; 조승찬; 길의준; 조상호; 박소윤; 이택견; 이석찬
KIOST Author(s)
Lee, Taek Kyun(이택견)
Publication Year
Red Sea bream Iridovirus (RSIV) is one of the most important marine viruses in Asia and infects high demanded fishes. RSIV spread rapidly in the sea around the world via various routes, such as ocean current, imported fishery products and ships’ ballast water discharged in ports. Therefore, the detection of RSIV is very important for food and economic security worldwide. Also, it is important for preservation of marine life. However, there are not any appropriate diagnostic methods for rapid diagnosis of marine viruses. In this study, scFv was used to solve this problem. Detection methods using scFvs are expected to show characteristics that more simplified, fast, specific and sensitive than ordinary antibodies-using methods. For selecting scFv recognizing target virus coat proteins, two display systems, phage display system and yeast surface display system, are used. First, viral coat proteins are expressed by yeast surface display which can express proteins with post-translational process. And then, scFv libraries, expressed by phage display, were screened against the viral coat proteins expressed by yeast for three rounds of panning. Finally, three scFvs are chosen for production as soluble antibody by E.coli and they will be used to develop the marine virus detection system. This strategy can be applied to other marine viruses threatening the health of ocean. Also it can provide easy and rapid virus specific scFv isolation and marine virus diagnosis application.
Bibliographic Citation
International Meeting of the Federation of Korean Microbiological Societies, pp.302, 2016
The Korean Sciety of Virology
The Korean Sciety of Virology
Related Researcher
Research Interests

Marine virus,Marine pathogens,해양바이러스,해양병원체

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