Dietary Exposed to Weathered Iranian Heavy Crude Oil : Effects on the detoxification and immune system in Rockfish (Sebastes schlegeli)

DC Field Value Language
dc.contributor.author 이은희 -
dc.contributor.author 정지현 -
dc.contributor.author 최광민 -
dc.contributor.author 김문구 -
dc.contributor.author 임운혁 -
dc.date.accessioned 2020-07-15T19:32:57Z -
dc.date.available 2020-07-15T19:32:57Z -
dc.date.created 2020-02-11 -
dc.date.issued 2016-11-09 -
dc.identifier.uri https://sciwatch.kiost.ac.kr/handle/2020.kiost/24351 -
dc.description.abstract This study examines whether acute toxic responses played by the immune system depend on which metabolic pathway was induced after exposure to weathered crude oil. We exposed juvenile rockfish to weathered crude oil (Iranian Heavy Crude Oil WIHCO in gelatin capsules) from the Hebei spirit oil spill by feeding. The effects on fish hepatodetoxification enzymes (Cytochrome P4501A) and the expression level of the immune response genes, including interleukin-1beta (IL-1?), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), Interferon-stimulated gene 15 (ISG 15) and granulocyte colony-stimulating factor (GCSF), were measured in the liver and kidney. The WIHCO-fed fish had significantly higher concentrations of biliary fluorescent metabolites and CYP1A expression during the initial stage after exposure (6 h after exposure) than those of the control group. Similarly, expression levels of IL-1?, p53, NF-kB and ISG15 mRNA were detected highly in the kidney at the early stages of exposure (6 h after exposure). In this study, the apoptosis, phagocytosis activity and cell cycle arrest analysis of juvenile rockfish exposed to WIHCO were measured by flow cytometry, a novel approach. Our results demonstrated that WIHCO caused an increase of apoptosis and cell cycle arrest and inhibition of phagocytic activity in juvenile Rockfish. Several immune-related genes in oil-fed fish were induced at the initial stage o WIHCO in gelatin capsules) from the Hebei spirit oil spill by feeding. The effects on fish hepatodetoxification enzymes (Cytochrome P4501A) and the expression level of the immune response genes, including interleukin-1beta (IL-1?), nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB), Interferon-stimulated gene 15 (ISG 15) and granulocyte colony-stimulating factor (GCSF), were measured in the liver and kidney. The WIHCO-fed fish had significantly higher concentrations of biliary fluorescent metabolites and CYP1A expression during the initial stage after exposure (6 h after exposure) than those of the control group. Similarly, expression levels of IL-1?, p53, NF-kB and ISG15 mRNA were detected highly in the kidney at the early stages of exposure (6 h after exposure). In this study, the apoptosis, phagocytosis activity and cell cycle arrest analysis of juvenile rockfish exposed to WIHCO were measured by flow cytometry, a novel approach. Our results demonstrated that WIHCO caused an increase of apoptosis and cell cycle arrest and inhibition of phagocytic activity in juvenile Rockfish. Several immune-related genes in oil-fed fish were induced at the initial stage o -
dc.description.uri 1 -
dc.language English -
dc.publisher Society of Environmental Toxicology and Chemistry -
dc.relation.isPartOf SETAC North America 37th Annual Meeting/7th SETAC World Congress -
dc.title Dietary Exposed to Weathered Iranian Heavy Crude Oil : Effects on the detoxification and immune system in Rockfish (Sebastes schlegeli) -
dc.type Conference -
dc.citation.conferencePlace US -
dc.citation.endPage 442 -
dc.citation.startPage 442 -
dc.citation.title SETAC North America 37th Annual Meeting/7th SETAC World Congress -
dc.contributor.alternativeName 이은희 -
dc.contributor.alternativeName 정지현 -
dc.contributor.alternativeName 김문구 -
dc.contributor.alternativeName 임운혁 -
dc.identifier.bibliographicCitation SETAC North America 37th Annual Meeting/7th SETAC World Congress, pp.442 -
dc.description.journalClass 1 -
Appears in Collections:
South Sea Research Institute > Risk Assessment Research Center > 2. Conference Papers
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