Rapid kit development for harmful algal detection
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Title
- Rapid kit development for harmful algal detection
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Author(s)
- 이나윤; 우선옥; 염승식
- KIOST Author(s)
- Woo, Seon Ock(우선옥); Yum, Seung Shic(염승식)
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Alternative Author(s)
- 이나윤; 우선옥; 염승식
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Publication Year
- 2016-11-10
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Abstract
- Invention of more quick, accurate, and efficient method which could have predictive and prognostic function is necessary to overcome the difficulties of conventional method for microorganisms monitoring. We have challenged to develop the immunochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H. pygmaea, A. sanguinea and C. polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C. closterium, and S. costatum. The rapid kit showed a positive signal at about 1,.000 cells of A tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.munochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H. pygmaea, A. sanguinea and C. polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C. closterium, and S. costatum. The rapid kit showed a positive signal at about 1,.000 cells of A tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/24342
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Bibliographic Citation
- 2016 대한환경 위해성 보건 과학회 학술대회, pp.10 - 34, 2016
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Publisher
- 대한환경 위해성 보건 과학회
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Type
- Conference
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Language
- English
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