Rapid kit development for harmful algal detection

Title
Rapid kit development for harmful algal detection
Author(s)
이나윤; 우선옥; 염승식
KIOST Author(s)
Lee, Na Yun(이나윤)Yum, Seungshic(염승식)
Publication Year
2016-11-10
Abstract
Invention of more quick, accurate, and efficient method which could have predictive and prognostic function is necessary to overcome the difficulties of conventional method for microorganisms monitoring. We have challenged to develop the immunochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H. pygmaea, A. sanguinea and C. polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C. closterium, and S. costatum. The rapid kit showed a positive signal at about 1,.000 cells of A tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.munochromatography based rapid kit for microalgae detection. As a result a rapid kit using monoclonal antibodies (mAbs) raised against RuBisCo (Ribulose-1.5-bisphosphate carboxylase /oxygenase) large subunit of Alexandrium tamarense, a candidate of harmful algal bloom species in the coast of Korea. The western blot analysis showed that the mAb detect five dinoflagellates, A. tamarense, H.circularisquama, H. pygmaea, A. sanguinea and C. polykrikoides. But do not detect H.triquetra, S.costatum, C.closterium. The mAb showed no signal in one dinoflagellate and two diatom species, C. closterium, and S. costatum. The rapid kit showed a positive signal at about 1,.000 cells of A tamarense and H.triquetra, 50,000 cells of A.sanguinea and so on.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24342
Bibliographic Citation
2016 대한환경 위해성 보건 과학회 학술대회, pp.10 - 34, 2016
Publisher
대한환경 위해성 보건 과학회
Type
Conference
Language
English
Publisher
대한환경 위해성 보건 과학회
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