Anti-oxidant activity of fucoidan isolated from Ecklonia cava against AAPH-induced oxidative stress in zebrafish model

Title
Anti-oxidant activity of fucoidan isolated from Ecklonia cava against AAPH-induced oxidative stress in zebrafish model
Author(s)
김은아; 전유진; 허수진
KIOST Author(s)
Kim, Eun A(김은아)Heo, Soo Jin(허수진)
Publication Year
2016-11-29
Abstract
Fucoidan, extracted from Ecklonia cava, has been extensively studied because of its wide biological activities. However, antioxidative activities have not been yet examined. Therefore we evaluated in vitro and in vivo studies on antioxidative activities of E. cava fucoidan (ECF). ECF exhibited more prominent effects in peroxyl radical scavenging activity, compared to the other scavenging activities. Thus, ECF was furher evaluated for its protective ability against 2,2-azobis dihydrochloride induced oxidative stress in Vero cells and ECF strongly reduced the AAPH-induced oxidative damage through scavenging intracellular reactive oxygen species. Furthermore, we evaluated protective effect of ECF against AAPH-induced oxidative stress in zebrafish model. ECF significantly reduced ROS generation, lipid peroxidation and cell death in zebrafish model. These findings indicate that ECF has antioxidant activities in vitro Vero cells and in vivo zebrafish model, even though ECF is not a polyphenol or flavonoid compound and does not contain benzene rings or conjugated structures.ve activities of E. cava fucoidan (ECF). ECF exhibited more prominent effects in peroxyl radical scavenging activity, compared to the other scavenging activities. Thus, ECF was furher evaluated for its protective ability against 2,2-azobis dihydrochloride induced oxidative stress in Vero cells and ECF strongly reduced the AAPH-induced oxidative damage through scavenging intracellular reactive oxygen species. Furthermore, we evaluated protective effect of ECF against AAPH-induced oxidative stress in zebrafish model. ECF significantly reduced ROS generation, lipid peroxidation and cell death in zebrafish model. These findings indicate that ECF has antioxidant activities in vitro Vero cells and in vivo zebrafish model, even though ECF is not a polyphenol or flavonoid compound and does not contain benzene rings or conjugated structures.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24279
Bibliographic Citation
한국해양바이오학회 정기총회 및 학술발표회, pp.147, 2016
Publisher
한국해양바이오학회
Type
Conference
Language
English
Publisher
한국해양바이오학회
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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