Distribution of dinoflagellate cysts in surface sediments of Tongyeong coastal waters, South Korea: Application of a next generation sequencing method
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Title
- Distribution of dinoflagellate cysts in surface sediments of Tongyeong coastal waters, South Korea: Application of a next generation sequencing method
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Author(s)
- 김현정; 정승원
- KIOST Author(s)
- Kim, Hyun Jung(김현정); Jung, Seung Won(정승원)
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Alternative Author(s)
- 김현정; 정승원
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Publication Year
- 2016-12-09
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Abstract
- To characterise the distribution of dinoflagellate cysts, we used next generation sequencing (NGS) after sampling of surface sediments of the Tongyeong coastal lines, South Korea. Sampling was divided into two seasons: a no red tide (52 sites, in December 2013) and a red tide (44 sites, in September 2014). The surface sediment samples were stored at 4 °C in a dark refrigerator for at least 6 months to allow decomposition of organic matter involving living phytoplankton. The NGS analysis revealed 5,270– 13,979 read counts and 175– 273 operational taxonomic units (OTUs) with 97% sequence similarity obtained after quality filtering of analysed sequences. Based on the NGS results, the Stramenopiles-Alveolata-Rhizaria (SAR) was the most abundant group (90.10% and 80.16% of the total analysed read counts of 2013 and 2014, respectively). The Alveolata represented the most dominant subgroup In Alveolata, the Dinophyta was the most abundant group (90.39% and 77.25% for 2013 and 2014, respectively). Identified species in the Dinophyta totalled 32 taxa consisting of 17 genera, 29 species, and 3 unidentified organisms. The presented common groups (classified at genus level), were Pentapharsodinium-Scrippsiella, Alexandrium, Gonyaulax, Gymnodinium, and Woloszynskia. The present result represents the first estimates of the diversity and species composition of dinoflagellate cysts that occur in the Tongyeong coastal sedies, in December 2013) and a red tide (44 sites, in September 2014). The surface sediment samples were stored at 4 °C in a dark refrigerator for at least 6 months to allow decomposition of organic matter involving living phytoplankton. The NGS analysis revealed 5,270– 13,979 read counts and 175– 273 operational taxonomic units (OTUs) with 97% sequence similarity obtained after quality filtering of analysed sequences. Based on the NGS results, the Stramenopiles-Alveolata-Rhizaria (SAR) was the most abundant group (90.10% and 80.16% of the total analysed read counts of 2013 and 2014, respectively). The Alveolata represented the most dominant subgroup In Alveolata, the Dinophyta was the most abundant group (90.39% and 77.25% for 2013 and 2014, respectively). Identified species in the Dinophyta totalled 32 taxa consisting of 17 genera, 29 species, and 3 unidentified organisms. The presented common groups (classified at genus level), were Pentapharsodinium-Scrippsiella, Alexandrium, Gonyaulax, Gymnodinium, and Woloszynskia. The present result represents the first estimates of the diversity and species composition of dinoflagellate cysts that occur in the Tongyeong coastal sedi
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/24260
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Bibliographic Citation
- 5th International Conference on Biotechnology and Bioengineering, pp.37 - 38, 2016
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Publisher
- ICCEMB
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Type
- Conference
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Language
- English
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