Cloning and Expression of a Putative Chitinase-Encoding Gene from a Thermophilic Marine Bacterium Rhodothermaceae sp. MEBiC09517

Title
Cloning and Expression of a Putative Chitinase-Encoding Gene from a Thermophilic Marine Bacterium Rhodothermaceae sp. MEBiC09517
Author(s)
박미정; 이경원; 안영준; 오지혜; 김병권; 권개경
KIOST Author(s)
Park, Mi-Jeong(박미정)Lee, Kyeong Won(이경원)Young, Jun An(안영준)Kwon, Kae Kyoung(권개경)
Alternative Author(s)
박미정; 이경원; 안영준; 오지혜; 권개경
Publication Year
2017-04-27
Abstract
Rhodothermaceae sp. MEBiC09517, a thermophilic marine bacteria with considerable evolutionary distance from previously reported, was isolated from a sediment near an wood processing company in Incheon City. Based on the completed genome data, a gene(ROT0835) among glycoside hydrolases affiliated with variety of GH families was selected. The ROT0835 has 1,140 nucleotides encoding 379 amino acids, and Mmolecular weight was predicted to be 42,354Da. The amino acid sequence showed identity with chitinase from Salinibacter ruber (56%) and glycoside hydrolase from Rhodothermus marinus (54%). ROT0835 was cloned and expressed through E.coli system using T-easy vector and pET-24a vector. An expressed protein was purified by affinity chromatography & size exclusion, and optimal condition for enzyme activity was estimated.a, a gene(ROT0835) among glycoside hydrolases affiliated with variety of GH families was selected. The ROT0835 has 1,140 nucleotides encoding 379 amino acids, and Mmolecular weight was predicted to be 42,354Da. The amino acid sequence showed identity with chitinase from Salinibacter ruber (56%) and glycoside hydrolase from Rhodothermus marinus (54%). ROT0835 was cloned and expressed through E.coli system using T-easy vector and pET-24a vector. An expressed protein was purified by affinity chromatography & size exclusion, and optimal condition for enzyme activity was estimated.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/24013
Bibliographic Citation
한국미생물학회 2017 국제학술대회, pp.268, 2017
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

Acquisition of marine microbial bioresources,Marine microbial diversity,Application study on marine microbes,해양미생물 자원 확보,해양미생물 다양성,해양미생물 기능 활용

Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse