Nisaea acidiphila sp. nov., Isolated from a Tropical Marine Algal Debris

Abstract

A Gram-negative, aerobic, rod-shaped and motile marine bacterium MEBiC11861T was isolated from a marine algal debris collected at Kosrae, Federation State of Micronesia. The 16S rRNA gene sequence analysis revealed that strain MEBiC11861T showed high similarity with members of the genus Nisaea (97.8-98.0%). Growth was observed at 10-42OC, at pH 4.0-8.5 and with 0-10% NaCl. The predominant cellular fatty acids are C12:0, C16:0, C12:0 3-OH, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and 8 (C18:1ω7c and/or C18:1ω6c), and C19:0 cyclo ω8c. The DNA G+C contents is 65.6 mol%. Several phenotypic characteristics such as utilization of gluconate, malate, adipate, arabinose etc., DNA G+C ratio, composition of cellular fatty acids, and growth range of pH and salinity differentiate strain MEBiC11861T from members of the genus Nisaea. Therefore, Nisaea acidiphila sp. nov. with the type strain MEBiC11861T (=KCCM 43046T =JCM 30369T) is proposed. Emended descriptions of the genus Nisaea Urios et al. 2008 is also given.howed high similarity with members of the genus Nisaea (97.8-98.0%). Growth was observed at 10-42OC, at pH 4.0-8.5 and with 0-10% NaCl. The predominant cellular fatty acids are C12:0, C16:0, C12:0 3-OH, summed feature 3 (C16:1ω7c and/or C16:1ω6c) and 8 (C18:1ω7c and/or C18:1ω6c), and C19:0 cyclo ω8c. The DNA G+C contents is 65.6 mol%. Several phenotypic characteristics such as utilization of gluconate, malate, adipate, arabinose etc., DNA G+C ratio, composition of cellular fatty acids, and growth range of pH and salinity differentiate strain MEBiC11861T from members of the genus Nisaea. Therefore, Nisaea acidiphila sp. nov. with the type strain MEBiC11861T (=KCCM 43046T =JCM 30369T) is proposed. Emended descriptions of the genus Nisaea Urios et al. 2008 is also given.