초고온 고세균에서의 다양한 베타-결합 다당류 대사관련 당화효소군의 동정

Abstract

The β-linked polysaccharides, such as cellulose, agarose, and laminarin, are major components of cell wall of macro algae such as green, red, and brown algae, which is an attractive biomass as a feedstock for producing biofuels and biochemical materials instead of crude oil. In this study, we identified novel glycoside hydrolase (GH) gene clusters from three Thermococcus strains (T. guaymasensis, T. pacificus, and T. waiotapuensis) by comparative genomic analysis compared to T. sibiricus. The clusters contain the two modular structures including glycoside hydrolases and cellobiose/β-glucoside ABC transporter, related to hydrolysis of various β-linked polysaccharides and transport of β-glycan oligomers into the cell, respectively. The glycoside hydrolases include cellulose-, laminarin-, and agarose-degrading enzymes. Furthermore, among of them, the GH cluster from T. pacificus was cloned into fosmid vector and the recombinant plasmid introduced into the genome of T. onnurineus NA1. The mutant strain could grow and H2 production on each carboxymethyl cellulose, laminarin, and agarose-containing medium.ical materials instead of crude oil. In this study, we identified novel glycoside hydrolase (GH) gene clusters from three Thermococcus strains (T. guaymasensis, T. pacificus, and T. waiotapuensis) by comparative genomic analysis compared to T. sibiricus. The clusters contain the two modular structures including glycoside hydrolases and cellobiose/β-glucoside ABC transporter, related to hydrolysis of various β-linked polysaccharides and transport of β-glycan oligomers into the cell, respectively. The glycoside hydrolases include cellulose-, laminarin-, and agarose-degrading enzymes. Furthermore, among of them, the GH cluster from T. pacificus was cloned into fosmid vector and the recombinant plasmid introduced into the genome of T. onnurineus NA1. The mutant strain could grow and H2 production on each carboxymethyl cellulose, laminarin, and agarose-containing medium.