초고온 고세균에서의 다양한 베타-결합 다당류 대사관련 당화효소군의 동정

Title
초고온 고세균에서의 다양한 베타-결합 다당류 대사관련 당화효소군의 동정
Alternative Title
Novel glycoside hydrolase gene clusters and hydrolysis of various β-linked polysaccharides in hyperthermophilic archaea
Author(s)
김윤재; 이규비; 이현숙; 강성균; 권개경; 이정현
KIOST Author(s)
Lee, Hyun Sook(이현숙)Kang, Sung Gyun(강성균)Kwon, Kae Kyoung(권개경)Lee, Jung Hyun(이정현)
Publication Year
2017-04-28
Abstract
The β-linked polysaccharides, such as cellulose, agarose, and laminarin, are major components of cell wall of macro algae such as green, red, and brown algae, which is an attractive biomass as a feedstock for producing biofuels and biochemical materials instead of crude oil. In this study, we identified novel glycoside hydrolase (GH) gene clusters from three Thermococcus strains (T. guaymasensis, T. pacificus, and T. waiotapuensis) by comparative genomic analysis compared to T. sibiricus. The clusters contain the two modular structures including glycoside hydrolases and cellobiose/β-glucoside ABC transporter, related to hydrolysis of various β-linked polysaccharides and transport of β-glycan oligomers into the cell, respectively. The glycoside hydrolases include cellulose-, laminarin-, and agarose-degrading enzymes. Furthermore, among of them, the GH cluster from T. pacificus was cloned into fosmid vector and the recombinant plasmid introduced into the genome of T. onnurineus NA1. The mutant strain could grow and H2 production on each carboxymethyl cellulose, laminarin, and agarose-containing medium.ical materials instead of crude oil. In this study, we identified novel glycoside hydrolase (GH) gene clusters from three Thermococcus strains (T. guaymasensis, T. pacificus, and T. waiotapuensis) by comparative genomic analysis compared to T. sibiricus. The clusters contain the two modular structures including glycoside hydrolases and cellobiose/β-glucoside ABC transporter, related to hydrolysis of various β-linked polysaccharides and transport of β-glycan oligomers into the cell, respectively. The glycoside hydrolases include cellulose-, laminarin-, and agarose-degrading enzymes. Furthermore, among of them, the GH cluster from T. pacificus was cloned into fosmid vector and the recombinant plasmid introduced into the genome of T. onnurineus NA1. The mutant strain could grow and H2 production on each carboxymethyl cellulose, laminarin, and agarose-containing medium.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23998
Bibliographic Citation
2017년도 한국미생물학회 국제학술대회 심포지엄, pp.1 - 2, 2017
Publisher
한국미생물학회
Type
Conference
Language
English
Publisher
한국미생물학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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