Angiotensin I-converting enzyme (ACE) inhibition and nitric oxide (NO)-mediated antihypertensive effect of octaphlorethol A isolated from Ishige sinicola: In vitro molecular mechanism and in vivo SHR model SCIE SCOPUS

Cited 16 time in WEB OF SCIENCE Cited 18 time in Scopus
Title
Angiotensin I-converting enzyme (ACE) inhibition and nitric oxide (NO)-mediated antihypertensive effect of octaphlorethol A isolated from Ishige sinicola: In vitro molecular mechanism and in vivo SHR model
Author(s)
Ko, Seok-Chun; Jung, Won-Kyo; Kang, Sung-Myung; Lee, Seung-Hong; Kang, Min Cheol; Heo, Soo-Jin; Kang, Kyong-Hwa; Kim, Yong-Tae; Park, Sun-Joo; Jeong, Yoonhwa; Kim, Misook; Byun, Hee-Guk; Jeon, You-Jin
KIOST Author(s)
Heo, Soo Jin(허수진)
Publication Year
2015-10
Abstract
Angiotensin l-converting enzyme (ACE) inhibition and nitric oxide (NO) production are important factors that regulate blood pressure. In this study, the effects of octaphlorethol A (OPA) isolated from Ishige sinicola on ACE inhibition and NO production, the molecular mechanism underlying ACE inhibition, as well as its antihypertensive effect in spontaneously hypertensive rats (SHRs) were investigated. IC50 value of OPA against ACE was 59 mu M. Molecular modelling studies indicated that the compound interacts with Cys370, Glu162, Glu376, Glu403, Glu411, Asp377, His383, His387, Tyr520, Arg522,Tyr523, and Lys511. In human endothelial cells, OPA increased endothelial nitric oxide synthase (eNOS) phosphorylation. We also demonstrated that these OPA-induced effects essentially depended on protein kinase B (Akt) and AMP-activated protein kinase (AMPK) activation. Furthermore, systolic blood pressure was reduced (21.9 mmHg in 6 h) by administration of the compound in SHRs. The results of this study suggested that OPA could be developed as a therapeutic agent for hypertension. (C) 2015 Elsevier Ltd. All rights reserved.
ISSN
1756-4646
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2398
DOI
10.1016/j.jff.2015.07.003
Bibliographic Citation
JOURNAL OF FUNCTIONAL FOODS, v.18, pp.289 - 299, 2015
Publisher
ELSEVIER
Subject
ECKLONIA-CAVA; BIOCHEMICAL-CHARACTERIZATION; ACTIVATION; PEPTIDE; HYDROLYSATE; FOLIACEA; AKT; PHOSPHORYLATION; VASORELAXATION; MELANOGENESIS
Keywords
Octaphlorethol A (OPA); Angiotensin I-converting enzyme (ACE); Nitric oxide (NO); Antihypertensive effect; Molecular mechanism
Type
Article
Language
English
Document Type
Article
Publisher
ELSEVIER
Related Researcher
Research Interests

Marine Biotechnology,Marine Natural Products,Bioactivities,해양생물공학,해양천연물학,생리활성

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