대사공학 기법을 이용한 초고온 고세균에서의 2,3-BDO 생산

Title
대사공학 기법을 이용한 초고온 고세균에서의 2,3-BDO 생산
Alternative Title
Production of 2,3-butanediol by metabolically engineered hyperthermophilic archaeon, Thermococcus onnurineus NA1
Author(s)
이규비; 임재규; 강성균; 이현숙; 이정현; 김윤재
KIOST Author(s)
Kang, Sung Gyun(강성균)Lee, Hyun Sook(이현숙)Lee, Jung Hyun(이정현)
Publication Year
2017-06-29
Abstract
2,3-butanediol (BDO) is a precursor for various plastics and pesticides. Many reports has been come out about biological 2,3-BDO production by mesophilic microorganisms. In hyperthermophiles, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from Pyrococcus furiosus. However, there are still no reports on 2,3-butnadiol production in hyperthermophiles. In this study, we first attempted to produce 2,3-BDO from hyperthermophilic archaeon, Thermococcus onnurineus NA1. These organism has no enzyme that turn out acetolactate, acetoin or 2,3-butanediol. So, we express two heterologous genes (acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from Thermococcus guaymacensis) to produce target metabolite in T. onnurineus NA1. The mutant strain produced about 4.0 g/L meso-form 2,3-BDO. In addition, to improve 2,3-BDO production, we have constructed acetyl-CoA synthetase III (TON_1001) deletion mutant and the mutant strain showed a 1.24-fold increase in 2,3-BDO productivity. Also, when carbon monoxide (CO) gas was added as a reductant, the 2,3-BDO productivity increased 1.25-fold.f 2,3-BDO, is produced from Pyrococcus furiosus. However, there are still no reports on 2,3-butnadiol production in hyperthermophiles. In this study, we first attempted to produce 2,3-BDO from hyperthermophilic archaeon, Thermococcus onnurineus NA1. These organism has no enzyme that turn out acetolactate, acetoin or 2,3-butanediol. So, we express two heterologous genes (acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from Thermococcus guaymacensis) to produce target metabolite in T. onnurineus NA1. The mutant strain produced about 4.0 g/L meso-form 2,3-BDO. In addition, to improve 2,3-BDO production, we have constructed acetyl-CoA synthetase III (TON_1001) deletion mutant and the mutant strain showed a 1.24-fold increase in 2,3-BDO productivity. Also, when carbon monoxide (CO) gas was added as a reductant, the 2,3-BDO productivity increased 1.25-fold.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23918
Bibliographic Citation
KMB 2017 국제학술대회 및 정기학술대회, pp.1 - 2, 2017
Publisher
한국미생물생명공학회
Type
Conference
Language
English
Publisher
한국미생물생명공학회
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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