대사공학 기법을 이용한 초고온 고세균에서의 2,3-BDO 생산
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Title
- 대사공학 기법을 이용한 초고온 고세균에서의 2,3-BDO 생산
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Alternative Title
- Production of 2,3-butanediol by metabolically engineered hyperthermophilic archaeon, Thermococcus onnurineus NA1
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Author(s)
- 이규비; 임재규; 강성균; 이현숙; 이정현; 김윤재
- KIOST Author(s)
- Lim, Jae Kyu(임재규); Kang, Sung Gyun(강성균); Lee, Hyun Sook(이현숙); Lee, Jung Hyun(이정현); Kim, Yun Jae(김윤재)
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Alternative Author(s)
- 임재규; 강성균; 이현숙; 이정현; 김윤재
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Publication Year
- 2017-06-29
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Abstract
- 2,3-butanediol (BDO) is a precursor for various plastics and pesticides. Many reports has been come out about biological 2,3-BDO production by mesophilic microorganisms. In hyperthermophiles, it has been reported that acetoin, a precursor of 2,3-BDO, is produced from Pyrococcus furiosus. However, there are still no reports on 2,3-butnadiol production in hyperthermophiles. In this study, we first attempted to produce 2,3-BDO from hyperthermophilic archaeon, Thermococcus onnurineus NA1. These organism has no enzyme that turn out acetolactate, acetoin or 2,3-butanediol. So, we express two heterologous genes (acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from Thermococcus guaymacensis) to produce target metabolite in T. onnurineus NA1. The mutant strain produced about 4.0 g/L meso-form 2,3-BDO. In addition, to improve 2,3-BDO production, we have constructed acetyl-CoA synthetase III (TON_1001) deletion mutant and the mutant strain showed a 1.24-fold increase in 2,3-BDO productivity. Also, when carbon monoxide (CO) gas was added as a reductant, the 2,3-BDO productivity increased 1.25-fold.f 2,3-BDO, is produced from Pyrococcus furiosus. However, there are still no reports on 2,3-butnadiol production in hyperthermophiles. In this study, we first attempted to produce 2,3-BDO from hyperthermophilic archaeon, Thermococcus onnurineus NA1. These organism has no enzyme that turn out acetolactate, acetoin or 2,3-butanediol. So, we express two heterologous genes (acetolactate synthase (alsS) from Pyrococcus sp. NA2 and alcohol dehydrogenase (adh) from Thermococcus guaymacensis) to produce target metabolite in T. onnurineus NA1. The mutant strain produced about 4.0 g/L meso-form 2,3-BDO. In addition, to improve 2,3-BDO production, we have constructed acetyl-CoA synthetase III (TON_1001) deletion mutant and the mutant strain showed a 1.24-fold increase in 2,3-BDO productivity. Also, when carbon monoxide (CO) gas was added as a reductant, the 2,3-BDO productivity increased 1.25-fold.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/23918
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Bibliographic Citation
- KMB 2017 국제학술대회 및 정기학술대회, pp.1 - 2, 2017
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Publisher
- 한국미생물생명공학회
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Type
- Conference
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Language
- English
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