Transcriptomic analysis in Javanicus medaka exposed to antidepressant

Abstract

Antidepressant drugs are mostly prescribed to treat symptoms of depression. These drugs are potentially influence living aquatic organisms. We investigated acute toxicity of Fluoxetine in Javanicus medaka (Oryzias javanicus) after 24, 48, 72 and 96 h of exposure. The LC50 values of Fluoxetine in O. javanicus after a specific period of exposure were 2.046±0.299 (24 h), 1.936±0.055 (48 h), 1.532±0.144 (72 h) and 1.237±0.160 mg/L (96 h). We also demonstrated transcriptomic analysis of O. javanicus exposed to Fluoxetine for 12, 24, 48 and 72 h. Hierarchical clustering of the differentially expressed genes showed similar gene’s profile after Fluoxetine exposure for 12, 24 and 48 h than that of 72 h. Functional annotation analysis showed various responses associated with hypoxia, oxidative stress, acute phase response and metabolic changes. Fluoxetine induces respiratory depression, leading to cell cycle arrest at G1/S phase. Oxidative stress associates with endoplasmic reticulum stress and protein degradation. Acute phase response indicates response to blood vessel injury. The changes in carbohydrate and lipid metabolisms associate with insulin resistance and Non-alcoholic fatty acid liver disease (NAFLD). These results extend our knowledge on toxic effects of human pharmaceutical in aquatic organisms.2 and 96 h of exposure. The LC50 values of Fluoxetine in O. javanicus after a specific period of exposure were 2.046±0.299 (24 h), 1.936±0.055 (48 h), 1.532±0.144 (72 h) and 1.237±0.160 mg/L (96 h). We also demonstrated transcriptomic analysis of O. javanicus exposed to Fluoxetine for 12, 24, 48 and 72 h. Hierarchical clustering of the differentially expressed genes showed similar gene’s profile after Fluoxetine exposure for 12, 24 and 48 h than that of 72 h. Functional annotation analysis showed various responses associated with hypoxia, oxidative stress, acute phase response and metabolic changes. Fluoxetine induces respiratory depression, leading to cell cycle arrest at G1/S phase. Oxidative stress associates with endoplasmic reticulum stress and protein degradation. Acute phase response indicates response to blood vessel injury. The changes in carbohydrate and lipid metabolisms associate with insulin resistance and Non-alcoholic fatty acid liver disease (NAFLD). These results extend our knowledge on toxic effects of human pharmaceutical in aquatic organisms.