Dynamic composition of the bacteria associated with Hydra magnipapillata exposed to Zinc oxide Nanoparticles

Abstract

Zinc oxide Nanoparticles (ZnO NP) have been widely used as additive materials for various applications. This condition may potentially affect to aquatic organisms and their associated microorganisms. This study aimed to investigate impact of ZnO NP to bacterial abundance and community composition, and changes in morphology of H. magnipapillata. A metagenomic approach with pyrosequencing 454 of the bacterial 16S rRNA gene and RDP pipeline tools was used to identify and classify bacterial community of H. magnipapillata. Morphological analysis was also used to identify morphological responses in H. magnipapillata after exposure of ZnO NP. The analysis revealed a significant increase of operational taxonomic units(OTUs) from 89 in unexposed polyps to 136, 150 and 156 OTUs in polyps exposed to ZnO NP for 24, 48 and 72 h, respectively. In polyps exposed to ZnO NP, a significant decrease in number of main associated bacteria was revealed, but bacteriawith beneficial functions were increased significantly. Gradual changes in morphology were observed in the polyps exposed to ZnO NP, suggesting specific toxic responses .f ZnO NP to bacterial abundance and community composition, and changes in morphology of H. magnipapillata. A metagenomic approach with pyrosequencing 454 of the bacterial 16S rRNA gene and RDP pipeline tools was used to identify and classify bacterial community of H. magnipapillata. Morphological analysis was also used to identify morphological responses in H. magnipapillata after exposure of ZnO NP. The analysis revealed a significant increase of operational taxonomic units(OTUs) from 89 in unexposed polyps to 136, 150 and 156 OTUs in polyps exposed to ZnO NP for 24, 48 and 72 h, respectively. In polyps exposed to ZnO NP, a significant decrease in number of main associated bacteria was revealed, but bacteriawith beneficial functions were increased significantly. Gradual changes in morphology were observed in the polyps exposed to ZnO NP, suggesting specific toxic responses .