saponification을 통해 sterol을 증가시킨 nannochloropsis oculata에 항염증, 항암 효과
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Title
- saponification을 통해 sterol을 증가시킨 nannochloropsis oculata에 항염증, 항암 효과
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Alternative Title
- Effect of increased sterol-rich fraction on anti-inflammatory and anti-cancer from Nannochloropsis oculata using saponification
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Author(s)
- 김준성; 최윤경; 예보람; 김은아; 허수진
- KIOST Author(s)
- Kim, Jun Seong(김준성); Kim, Eun A(김은아); Heo, Soo Jin(허수진)
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Alternative Author(s)
- 김준성; 최윤경; 예보람; 김은아; 허수진
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Publication Year
- 2017-11-17
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Abstract
- The green microalga of the genus Nannochloropsis (class Eustigmatophyceae) is a leading candidate for biofuel production due to its ability to accumulate high oil content (28.7% of cellular ash-free dry weight). We investigated the anti-inflammatory and anticancer activities of sterol-rich fraction from Nannochloropsis oculata n-hexane (NOH) extract after saponification of the microalga. Among the fractions with n-hexane, chloroform and ethyl acetate, the n-hexane fraction showed the highest anti-inflammatory activity in LPS-stimulated RAW 264.7 macrophage as well as anticancer activity against human leukemia HL-60 cells without the cytotoxicity. And the sterol-rich fraction was obtained from the n-hexane fraction by open silica column under the gradient solvent condition with 100% hexane (1L), hexane : ethyl acetate (20 : 1, 10 : 1, 5 : 1, 1 : 1, v/v). Among the four fraction (NOH-1~4), especially NOH-1 contained the highest content of sterols. NOH1 shoed the highest HL-60 (about 85%) and NO inhibitory activities at the concentration of 100 μg/mL. These results demonstrated that the sterol-rich fraction from N. oculata might be a useful candidate as anti-inflammatory and anticancer agents for anti-inflammatory and anticancer activity.lammatory and anticancer activities of sterol-rich fraction from Nannochloropsis oculata n-hexane (NOH) extract after saponification of the microalga. Among the fractions with n-hexane, chloroform and ethyl acetate, the n-hexane fraction showed the highest anti-inflammatory activity in LPS-stimulated RAW 264.7 macrophage as well as anticancer activity against human leukemia HL-60 cells without the cytotoxicity. And the sterol-rich fraction was obtained from the n-hexane fraction by open silica column under the gradient solvent condition with 100% hexane (1L), hexane : ethyl acetate (20 : 1, 10 : 1, 5 : 1, 1 : 1, v/v). Among the four fraction (NOH-1~4), especially NOH-1 contained the highest content of sterols. NOH1 shoed the highest HL-60 (about 85%) and NO inhibitory activities at the concentration of 100 μg/mL. These results demonstrated that the sterol-rich fraction from N. oculata might be a useful candidate as anti-inflammatory and anticancer agents for anti-inflammatory and anticancer activity.
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URI
- https://sciwatch.kiost.ac.kr/handle/2020.kiost/23572
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Bibliographic Citation
- The role of food scientists & food industries for food security, pp.11 - 66, 2017
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Publisher
- Korean
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Type
- Conference
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Language
- English
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