Extraction and characterization of pectin-like polysaccharide from marine algae

Abstract

Pectin and/or pectin-like polysaccharide (PLP) is one of complex polysaccharides contained in the plant cell walls, consisted of galacturonic acid polymer as a backbone and neutral sugars chained on branches. Pectin is widely used as a functional ingredient in the food industry as gelling and stabilizing polymers. Recently, Pectin has on important role in removal of heavy metals, and antioxidative activity of PLP reported on beneficial for human health. The aim of this study was to extract PLP from various marine algae to establish optimum extract condition, and to prove the efficacy as fundamental material for human health. PLP is extracted from 5 kinds of microalgae and 9 kinds of macroalgae with different extraction methodologies. High yield of PLP was extracted in distilled water (DW) as 21.06±3.5% from A. maxima. PLP yield from macroalgae was lower than microalgae. In acidic condition (AC), PLP from Undaria pinnatifida was not precipitated. However, the yields of galacturonic acid was higher in Hizikia fusiformis (80.28±4.58%) and Laminaria japonica (65.85±0.61%), respectively. In biological activity tests, Fe2+ chelating activity was 26% higher than pectin from citrus peel as control. ABTS scavenging assay showed 100% antioxidative activity based on DW extraction from Ecklonia cava, whereas not detected from other macroalgae. This study suggested that further study of functional properties of PLP should be ctional ingredient in the food industry as gelling and stabilizing polymers. Recently, Pectin has on important role in removal of heavy metals, and antioxidative activity of PLP reported on beneficial for human health. The aim of this study was to extract PLP from various marine algae to establish optimum extract condition, and to prove the efficacy as fundamental material for human health. PLP is extracted from 5 kinds of microalgae and 9 kinds of macroalgae with different extraction methodologies. High yield of PLP was extracted in distilled water (DW) as 21.06±3.5% from A. maxima. PLP yield from macroalgae was lower than microalgae. In acidic condition (AC), PLP from Undaria pinnatifida was not precipitated. However, the yields of galacturonic acid was higher in Hizikia fusiformis (80.28±4.58%) and Laminaria japonica (65.85±0.61%), respectively. In biological activity tests, Fe2+ chelating activity was 26% higher than pectin from citrus peel as control. ABTS scavenging assay showed 100% antioxidative activity based on DW extraction from Ecklonia cava, whereas not detected from other macroalgae. This study suggested that further study of functional properties of PLP should be c