Gene expression changes in Fish Oryzias javanicus exposed to Fluoxetine

Abstract

Fluoxetine is one of the most prescribed drugs to treat depression, panic, anxiety, or obsessive-compulsive symptoms. The effects of this drug were investigated in O. javanicus to predict biological processes associated with toxic responses in aquatic organism. The median lethal concentrations (LC50) of Fluoxetine in O. javanicus were 2.046±0.299 (24 h), 1.936±0.055 (48 h), 1.532±0.144 (72 h) and 1.237±0.160 mg/L (96 h). Transcriptomic analysis showed dynamic changes in the differentially expressed genes of O. javanicus exposed to Fluoxetine for 12, 24, 48 and 72 h. Functional annotation of the differentially expressed genes suggested that these genes were involved in respiratory depression associated with cell cycle arrest, oxidative stress inducing endoplasmic reticulum stress and protein damage, acute phases response associated with inflammatory responses and metabolic changes associated with changes in carbohydrate and lipid metabolisms. The genes associated with locomotory behavior were also affected in the exposed fish. Thus, this study extends understanding in toxic effect of human pharmaceutical product in environment. in aquatic organism. The median lethal concentrations (LC50) of Fluoxetine in O. javanicus were 2.046±0.299 (24 h), 1.936±0.055 (48 h), 1.532±0.144 (72 h) and 1.237±0.160 mg/L (96 h). Transcriptomic analysis showed dynamic changes in the differentially expressed genes of O. javanicus exposed to Fluoxetine for 12, 24, 48 and 72 h. Functional annotation of the differentially expressed genes suggested that these genes were involved in respiratory depression associated with cell cycle arrest, oxidative stress inducing endoplasmic reticulum stress and protein damage, acute phases response associated with inflammatory responses and metabolic changes associated with changes in carbohydrate and lipid metabolisms. The genes associated with locomotory behavior were also affected in the exposed fish. Thus, this study extends understanding in toxic effect of human pharmaceutical product in environment.