FGF11은 저산소 상태에서 HIF-1a 안정성 증진 인자로써의 역할을 한다

Title
FGF11은 저산소 상태에서 HIF-1a 안정성 증진 인자로써의 역할을 한다
Alternative Title
FGF11 acts as a stabilizer of HIF-1alpha under hypoxic condition
Author(s)
이경원; 임형순; 신지혜; 이철주; 이정현; 정재연
KIOST Author(s)
Lee, Kyeong Won(이경원)Yim, Hyung Soon(임형순)Lee, Jung Hyun(이정현)
Publication Year
2018-03-28
Abstract
Fibroblast growth factor 11 (FGF11) is a member of intracellular FGFs. Although induction of FGF11 by hypoxia has been observed in several cell types, molecular function of FGF11 is not clearly understood yet. This study aimed to investigate the role of FGF11 under hypoxia. Immunoprecipitation and mass spectrometry analysis showed that many interacting partners for FGF11 are related with ubiquitination regulatory mechanism. And we found hypoxia-inducible factors-1 (HIF-1a) as a binding partner of FGF11. FGF11 knockdown decreased HIF-1a protein, while FGF11 overexpression increased it, without affecting HIF-1a mRNA. FGF11 knockdown increased ubiquitination levels of HIF-1a and decreased its stability, while FGF11 overexpression decreased ubiquitination levels of HIF-1a and increased its stability. These results suggest a cross-regulation between HIF-1a and FGF11, through which hypoxia-induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF-1a.e the role of FGF11 under hypoxia. Immunoprecipitation and mass spectrometry analysis showed that many interacting partners for FGF11 are related with ubiquitination regulatory mechanism. And we found hypoxia-inducible factors-1 (HIF-1a) as a binding partner of FGF11. FGF11 knockdown decreased HIF-1a protein, while FGF11 overexpression increased it, without affecting HIF-1a mRNA. FGF11 knockdown increased ubiquitination levels of HIF-1a and decreased its stability, while FGF11 overexpression decreased ubiquitination levels of HIF-1a and increased its stability. These results suggest a cross-regulation between HIF-1a and FGF11, through which hypoxia-induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF-1a.
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23454
Bibliographic Citation
Fibroblast Growth Factors in Development and Disease, 2018
Publisher
Gordon
Type
Conference
Language
English
Publisher
Gordon
Related Researcher
Research Interests

marine biotechnology,molecular microbiology,해양생명공학,분자미생물학

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