Genomic Properties of the Novel Natural Products producing Marine Bacterium Streptomyces sp. MEBiC10311

Abstract

Genome of a Streptomyces strain was sequenced with PacBio RSII system SMRT sequencing technology and its genes related to the production of secondary metabolites were investigated via anti-SMASH web-based tool. Strain MEBiC10311 showed no difference with S. rutgersensis, S. gougerotii, and S. diabolicus in 16S rRNA gene sequence similarity, however, genome of strain MEBiC10311 was different from previously known strains with species level. Genome of strain MEBiC10311 was 7.28 Mbps size, 73.3 mol% of DNA G+C ratio, 5,882 CDS, and include 14 gene clusters related to the production of secondary metabolites. The clusters consisted with four NRPS, one type 1 PKS, 1 siderophore, and 7 PKS-NRPS hybrids. Among the 14 predicted products, 11 were novel from previously known compounds. This study revealed that small differences in the genome made novel differences in the production of natural products. [Supported grants from KIOST & MBRB].ifference with S. rutgersensis, S. gougerotii, and S. diabolicus in 16S rRNA gene sequence similarity, however, genome of strain MEBiC10311 was different from previously known strains with species level. Genome of strain MEBiC10311 was 7.28 Mbps size, 73.3 mol% of DNA G+C ratio, 5,882 CDS, and include 14 gene clusters related to the production of secondary metabolites. The clusters consisted with four NRPS, one type 1 PKS, 1 siderophore, and 7 PKS-NRPS hybrids. Among the 14 predicted products, 11 were novel from previously known compounds. This study revealed that small differences in the genome made novel differences in the production of natural products. [Supported grants from KIOST & MBRB].