Revealing the Hybrid Zone and Genetic Polymorphisms within Pseudo-nitzschia pungens the Cosmopolitan Diatom Species

Title
Revealing the Hybrid Zone and Genetic Polymorphisms within Pseudo-nitzschia pungens the Cosmopolitan Diatom Species
Author(s)
김진호; Pengbin Wang; 박범수; 김주환; Shailesh Kumar Patidar; Penelope Ajani; Shauna Murray; Hong Chang Lim; Po Teen Lim; 한명수
Publication Year
2018-04-27
Abstract
Genetic sub-populations (clades) of cosmopolitan marine diatom Pseudo-nitzschia pungens might have distinct habitats, and their hybrid zone is suspected in the West Pacific area, however, it is still unrevealed because of technical difficulties and lack of evidences in nature. We employed the 137 number of genetic information of P. pungens and its sampling data originated from the West Pacific area, and used field application of qPCR assay for intra-specific level of P. pungens. Only two genotypes, clade I and III, were considered in the West Pacific area. Clade I was distributed from 39 to 32.3 °N, and clade III were from 1.4 to 34.4 °N. The estimated habitat temperature for the clade I and clade III rangeswere 8.1 - 26.9 °C and 24.2 - 31.2 °C, respectively. The latitudal distributions and habitat temperature ranges of each clade were significantly different, however, the results of qPCR assay revealed that their hybrid has being created in the southern Korean coasts. In laboratory condition, the sexual-reproduction was successfully occurred, and offspring cells were produced between clade I and III strains. The offspring strains showed recombinant sequences types, and the high ratios (38.6 and 65.1%) of single nucleotide polymorphism were observed in ITS2 region. The hybrid zone and genetic polymorphism phenomenon should contribute to maintain the genetic diversity and to produce the new genotypes within this
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23404
Bibliographic Citation
응용생태공학회 환경생물학회 공동학술대회, pp.1, 2018
Publisher
한국환경생물학회
Type
Conference
Language
English
Publisher
한국환경생물학회
Files in This Item:
There are no files associated with this item.

qrcode

Items in ScienceWatch@KIOST are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse