Polymorphic microsatellite markers for the endangered fish, the slender shiner Pseudopungtungia tenuicorpa and cross-species amplification across five related species SCIE SCOPUS

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Title
Polymorphic microsatellite markers for the endangered fish, the slender shiner Pseudopungtungia tenuicorpa and cross-species amplification across five related species
Author(s)
Kim, K. S.; Moon, S. J.; Han, S. H.; Kim, K. Y.; Bang, I. C.
Alternative Author(s)
김근식
Publication Year
2016
Abstract
The slender shiner Pseudopungtungia tenuicorpa (Cypriniformes; Cyprinidae; Gobioninae) is an endangered freshwater fish species endemic to Korea. The current strategies for its conservation involve the study of population genetic characters and identification of management units. These strategies require suitable molecular markers to study genetic diversity and genetic structure. Here, we developed nine polymorphic microsatellite markers for P. tenuicorpa for the first time by applying an enrichment method from a size-selected genomic library. The developed microsatellite markers produced a total of 101 alleles (average 11.2). The observed and expected heterozygosities averaged 0.805 and 0.835, respectively. Among the nine identified markers, five markers showed successful amplification across five related Korean Gobioninae species. Thus, the microsatellite markers developed in this study will be useful to establish conservation strategies for both P. tenuicorpa and other related species.
ISSN
1676-5680
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2325
DOI
10.4238/gmr.15038496
Bibliographic Citation
GENETICS AND MOLECULAR RESEARCH, v.15, no.3, 2016
Publisher
FUNPEC-EDITORA
Subject
FRESH-WATER; SOFTWARE
Keywords
Pseudopungtungia tenuicorpa; Microsatellite; Endangered species; Genetic diversity; Cross-species amplification; Conservation
Type
Article
Language
English
Document Type
Article
Publisher
FUNPEC-EDITORA
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