Assessing Coral Stress Responses Using Microarray

Title
Assessing Coral Stress Responses Using Microarray
Author(s)
우선옥; 염승식
KIOST Author(s)
Yum, Seungshic(염승식)
Publication Year
2018-07-23
Abstract
This study focused on the identification of functional genes in coral to assess the molecular level responses to environmental stresses like as seawater temperature change and hypoxia and marine acidification. Major interests are the marine environmental changes and marine organisms in relation to molecular level stress responses and various environmental stresses. Climate change and rising seawater temperature are placing spatially divergent stresses on the worlds coral ecosystem through increasing ocean surface temperatures and ocean acidification. To assess the coral health condition as well as coral stress responses, we purposed to identify molecular level stress responses using gene expression change as well as functional genes responding to external stress like increased seawater temperature in this study. We extracted RNA from soft coral, Scleronephthya gracillimum and carried out RNA sequencing using NGS method. S. gracilimum microarray was made by unigenes after NGS assembly and blast search. Approximately 38000 genes were uploaded in S.gracillimum microarray. We carried out temperature stress experiment using soft coral then extracted RNA for microarray experiment. In this research, we investigated transcriptional responses of S. gracillimum against heat shock. As the results, we found several functional groups of genes from coral responding to high seawater temperature. Approximately 200 genes including environmental changes and marine organisms in relation to molecular level stress responses and various environmental stresses. Climate change and rising seawater temperature are placing spatially divergent stresses on the worlds coral ecosystem through increasing ocean surface temperatures and ocean acidification. To assess the coral health condition as well as coral stress responses, we purposed to identify molecular level stress responses using gene expression change as well as functional genes responding to external stress like increased seawater temperature in this study. We extracted RNA from soft coral, Scleronephthya gracillimum and carried out RNA sequencing using NGS method. S. gracilimum microarray was made by unigenes after NGS assembly and blast search. Approximately 38000 genes were uploaded in S.gracillimum microarray. We carried out temperature stress experiment using soft coral then extracted RNA for microarray experiment. In this research, we investigated transcriptional responses of S. gracillimum against heat shock. As the results, we found several functional groups of genes from coral responding to high seawater temperature. Approximately 200 genes including
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/23181
Bibliographic Citation
European congress of Biotechnology, pp.28, 2018
Publisher
European Congress of Biotechnology 18th
Type
Conference
Language
English
Publisher
European Congress of Biotechnology 18th
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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