cDNA and gene structures of two phospholipase A2 isoforms, acidic PLA2 PA4 and PLA2 PA3A/PA3B/PA5, in Nemopilema nomurai jellyfish venom SCIE SCOPUS

Cited 4 time in WEB OF SCIENCE Cited 4 time in Scopus
Title
cDNA and gene structures of two phospholipase A2 isoforms, acidic PLA2 PA4 and PLA2 PA3A/PA3B/PA5, in Nemopilema nomurai jellyfish venom
Author(s)
Heo, Y.; Kwon, Y.C.; Shin, K.; Yoon, W.D.; Han, C.H.; Yum, S.; Kim, E.
KIOST Author(s)
Shin, Kyoungsoon(신경순)Yum, Seungshic(염승식)
Publication Year
2016
Abstract
We have shown that Nemopilema nomurai jellyfish venom (NnV) contains various kinds of proteolytic enzyme activities, including phospholipase (PLA), metalloproteinase (MP) and hyaluronidase activities. In this study, we reported the full-length cDNA and gene sequences of two PLA2 isoforms: acidic PLA2 PA4 and PLA2 PA3A/PA3B/PA5. The full-length cDNA of acidic PLA2 PA4 contains 483 nucleotides (nt), which encode 160 amino acids (and the stop codon), including a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The gene sequence of the acidic PLA2 PA4 is 1667 base pairs (bp) long and encodes three exons and two introns. The 5′ donor (GT) and 3′ acceptor (AG) splice sites are highly conserved. The PLA2 PA3A/PA3B/PA5 gene contains 1366 bp, and the 498 nt of the mature mRNA encode 165 amino acids (and the stop codon). The protein includes a signal peptide, six cysteine residues that form disulfide bonds, and metal-binding and catalytic active sites. The three exons and two introns also have highly conserved donor and acceptor splice sites. InterProScan predicted PLA2 activity domains in both isoforms. These results extend our understanding of the PLA2 venom of the N. nomurai jellyfish and will facilitate further research. © 2016 Elsevier Ltd
ISSN
0041-0101
URI
https://sciwatch.kiost.ac.kr/handle/2020.kiost/2313
DOI
10.1016/j.toxicon.2016.10.006
Bibliographic Citation
Toxicon, v.122, pp.160 - 166, 2016
Publisher
Elsevier Ltd
Subject
complementary DNA; cysteine; esterase; genomic DNA; jellyfish venom; messenger RNA; phospholipase A2 PA3A; phospholipase A2 PA3B; phospholipase A2 PA4; phospholipase A2 PA5; signal peptide; unclassified drug; venom; coelenterate venom; complementary DNA; phospholipase A2; animal tissue; Article; base pairing; controlled study; disulfide bond; enzyme active site; exon; gene sequence; gene structure; intron; jellyfish; nematocyst; Nemopilema nomurai; nonhuman; prediction; priority journal; protein domain; RNA splicing; stop codon; Cnidarian Venoms; DNA, Complementary; Phospholipases A2
Keywords
Acidic PLA2 PA4; Full-length cDNA sequence; Genomic DNA sequence; Nemopilema nomurai jellyfish venom; Phospholipase A2; PLA2 PA3A/PA3B/PA5
Type
Article
Language
English
Document Type
Article
Publisher
Elsevier Ltd
Related Researcher
Research Interests

Marine Genomics,Environmental Genomics,Novel biomaterials,해양생물유전체,생태환경유전체,바이오신소재

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